Structural analysis of carbohydrates

PROTON SPIN-LATTICE RELAXATION RATES IN THE STRUCTURAL ANALYSIS OF CARBOHYDRATE MOLECULES... [Pg.125]

Proton Spin—Lattice Relaxation Rates in the Structural Analysis of Carbohydrate Molecules in Solution... [Pg.405]

Vliegenthart, J.F.G., Dorland, L., and van Halbeek, H. (1983) High-resolution, lH-nuclear magnetic resonance spectroscopy as a tool in the structural analysis of carbohydrates related to glycoproteins. Adv. Carbohydr. Chem. Biochem. 41, 209-374. [Pg.1125]

The present article deals with the applications of mass spectrometry to the structural analysis of carbohydrate derivatives. The mass-spectral technique has now become a useful supplement to chemical methods, and provides ready solution of a variety of problems which were a stumbling block in the classical approaches. [Pg.39]

The structural analysis of carbohydrates, which is the major interest in the interpretation of their vibrational spectra, necessitates making a synthesis of the information given by different techniques. It may be seen from Scheme 1 that the determination of a structure lies at the crossroads of different kinds of information. [Pg.11]

HIGH-RESOLUTION, 1H-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AS A TOOL IN THE STRUCTURAL ANALYSIS OF CARBOHYDRATES RELATED TO GLYCOPROTEINS... [Pg.209]

I. Ciucanu, Per-O-methylation reaction for structural analysis of carbohydrates by mass spectrometry, Anal. Chim. Acta, 576 (2006) 147-155. [Pg.195]

An excellent set of practical protocols in use today is given by R. Stenitz, P.-E. Jansson and G. Widmalm, A Practical Guide to the Structural Analysis of Carbohydrate, http //www.casper.organ.su.se. [Pg.281]

The use of mass spectrometry in the structural analysis of carbohydrates, first reported in 1958 (114), was developed in detail by Kochetkov and Chizhov (115). They showed that, under electron impact, the acetylated and methyl ether derivatives of monosaccharides provided a wealth of structural information through analysis of typical fragmentation pathways of the initial molecular ion. This has proved of enormous utility in the structural elucidation of polysaccharides and complex oligosaccharides sequential permethylation, hydrolysis, reduction to the alditol, and acetylation, affords mixtures of peracetylated, partially methylated alditol acetates that can be separated and analyzed by use of a gas chromatograph coupled directly to a mass spectrometer (25). The mass spectra of stereoisomers are normally identical, while the gas chromatographic retention times readily permit differentiation of stereoisomers. [Pg.17]

MS is an important tool for the structural analysis of carbohydrates and offers precise results, analytical versatility, and very high sensitivity. Whereas MS analysis options for proteins and peptides are well defined relative to those for carbohydrates, tandem MS product-ion patterns are more complex and the pathways are still being elucidated. [Pg.44]

Characteristic differences in the n.m.r. chemical shifts of the mono- and di-O-methyl derivatives of methyl 2-acetamido-2-deoxy-D-glucopyranoside have been observed and should be of value in the structural analysis of carbohydrate chains containing these residues. ... [Pg.244]

This reaction has wide application at earlier stages of carbohydrate chemistry, for the sake of structural analysis of carbohydrates. [Pg.1339]

C-n.m.r. spectroscopy and its application in carbohydrate chemistry and on 2D n.m.r. techniques for structure analysis of carbohydrates have appeared. [Pg.226]

Procedures for etherification and esterification of carbohydrates for GLC analysis, advantages and disadvantages of the different methods of hydroxyl and aldehyde group derivatization, columns used for the separation of the various derivatives, detection methods for GLC, mass spectroscopy and fast atom bombardment (FAB) as well as outlines of some strategies for structural analysis of carbohydrates are described, discussed and reviewed in an excellent book on the analyses of carbohydrates by GLC (35). [Pg.145]

Also, they are used for the structural analysis of carbohydrates (117). The information obtained is not limited to that obtained by analysis of the hydrolysis fragments because the specificity of enzyme action, a specificity based on type of monosaccharide and type of linkage, leads to significant data being obtained, by a process of elimination, from enzyme resistant structures and partially hydrolyzed structures. The enzymes which hydrolyse polysaccharides are divided into two groups, endo- and exo-polysaccharide hydrolases. Endo- polysaccharide hydrolases are specific for linkage and... [Pg.165]

Recent advances in mass spectrometric instrumentation and methodology have been reviewed within the context of the structural analysis of carbohydrates. A review (16 refs, in Chinese) on the application of fast atom bombardment (FAB)-m.s. to the analysis of 0-glycosides has appeared. ... [Pg.276]

Reviews have been published on the use of spin-lattice relaxation rates in the structural analysis of carbohydrate molecules in solution" and on the application of 2D n.m.r. spectroscopy to the structural analysis of oligosaccharides and other complex carbohydrates. ... [Pg.228]

The principles and use of dlrect/desorption c.l.-m.s. for the structural analysis of carbohydrates and oligosaccharides have been reviewed (5 refs.). ... [Pg.234]

This is important because structure analysis of carbohydrates requires significant quantities of glycoconjugates. The availability of material adequate for quantitative analysis of biological activities is also very important for studying structure-function relationships. The specific activities of the putative interacting molecules... [Pg.1989]

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