Streptomyces halstedii

A selected strain o1 Streptomyces halstedii was cultivated in an aqueous nutrient medium under aerobic conditions and the resulting broth containing carbomycin antibiotics was filtered. The solutions was extracted twice at pH 6.5 with one-quarter volume of methyl isobutyl ketone. The combined extracts were concentrated to one-tenth volume under vacuum, and the antibiotics were extracted into water adjusted to a pH of about 2 with sulfuric acid. After adjusting the separated aqueous solution to pH 6.5, the antibiotic was extracted into benzene and the solution was concentrated to a small volume. Addition of hexane resulted in the separation of a solid product containing the benzene complexes of carbomycin A and carbomycin B, present in the fermentation broth. 

Streptomycesgriseus No. 3570 Ca ndicldin Streptomyces griseus Streptomycin Streptomyces halstedii Carbomycin... 

Carbomycin A Carbomycin A P1 Streptomyces halstedii Streptomyces lutea and... 

The microorganism Streptomyces halstedii elaborates an antibiotic substance, called carbomycin32 or Magnamycin,33 which has been isolated from the fermentation broth by simple, solvent extraction, and then purified by recrystallization. (Examination of the resulting mother liquors has led to the isolation of a closely similar compound designated as Magnamycin B. 34) Carbomycin has low toxicity,85 and appears to be an effective and satis-... 

Ogasawara Y, Katayama K, Minami A et al (2004) Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. Chem Biol 11 79-86... 

Ruiz-Arribas, A., Fernandez-Abalos, J. M., Sanchez, P., Garda, A. L., and Santamaria, R. I., Overproduction, purification, and biochemical characterization of a xylanase (Xysl) from Streptomyces halstedii JM8. Appl Environ Microbiol 1995, 61 (6), 2414-9. 

CAS 4564-87-8. C42H67N016. An antibiotic isolated from products of Streptomyces halstedii when grown in suitable media by the deep-culture method. It inhibits growth of certain Gram-positive bacteria such as staphylococci, pneumococci, and hemolytic streptococci. Mp 214C. 

Figure 4 Partial organization of the poiyketide synthase (PKS) system for which AT domain substrate specificity and coiinearity ruie-based prediction has ied to the discovery of the haistoctacosanoiides. AT domains highiighted in green were predicted to specificaiiy ioad a methyimaionyi-CoA unit whereas the ones highiighted in biue were predicted to ioad maionyi-CoA. The actuai structure of the haistoctacosanoiides isoiated from Streptomyces halstedii is represented in the bottom ieft corner.

Adham, S.A., Campelo, A.B., Ramos, A., and Gil, J.A. (2001) Construction of a xylanase-producing strain of Brevibacterium lactofermentum by stable integration of an engineered xysA gene from Streptomyces halstedii JM8. Appl. Environ. Microbiol, 67 (12), 5425 - 5430. 

A7-3, 73376-48A] Prod, by Streptomyces halstedii and Streptomyces aburaviertsis rufus. Active against gram-negative bacteria and fungi. 

C37H59NO15 757.871 Prod, by Streptomyces halstedii deltae. Okamura, K. et al, J. Ferment Technol, 1977, 55, 347 isol)... 

This peptidolipid has been found in four species of Actinomycetales, Streptomyces halstedii, Streptoverticillium cinnamoneum, Streptomyces... 

Hochstein, F. a., and K. Murai Magnamycin B, a second antibiotic from Streptomyces halstedii. J. Am. Chem. Soc. 76, 508O (1954). 

---