Strategy for Scaling-up of SEC

Sample volume to be processed/cycle (L) Recommended Sephadex G-type Recommended column type Bed height (cm) Bed volume (L) Pump type Flow-rate range (Lh- ) Approximate cycle time (min) 

Maximum column loading depends on the application and requirement. Generally, sample size should not exceed 20% of column volume. A pump Sera 41 L (Seybert and Rahier, D-3524 Immenhausen, Germany) is required for packing. 

All BPSS columns will operate at flow-rate up to 5mLcm h.  

It was in 1964 that Moore suceessfully synthesised a macroporous poly(styrene/ divinylbenzene) copolymer whieh enabled polymers to be utilised for liquid chromatography without the restrictions of operating pressure previously imposed [3]. The rigidity and controlled pore size and strueture of these polymer matrices has enabled the development of high speed, high performanee separations. 

Porous polymeric adsorbents for liquid chromatography can be categorised as being either microporous of macroporous depending upon the morphology of the individual particle. The type of pore structure is dependent upon the degree of cross-linking of the polymer chains within each particle. 

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