Stimulants monitoring

Oldiges, M., Kunze, M., Degenring, D. et al. (2004) Stimulation, monitoring, and analysis of pathway dynamics by metabolic profiling in the aromatic amino acid pathway. Biotechnology Progress, 20, 1623-1633. 

Cocaine and other CNS stimulants Monitor cardiac function Lorazepam 2-4 mg IM every 30 minutes to 6 hours as needed for agitation Haloperidol 2-5 mg (or other antipsychotic agent) every 30 minutes to 6 hours as needed for psychotic behavior B2 B3... 

Vigilance ability—Typically, control room operators are required to engage in lengthy, low-stimulation monitoring tasks and because of this understimulation can occur. These situations require a vigilance ability on behalf of the operator, which refers to the ability to maintain a form of alertness despite the soporific nature of the woik. 

Laser stimulation of a silver surface results in a reflected signal over a million times stronger than that of other metals. Called laser-enhanced Raman spectroscopy, this procedure is useful in catalysis. The large neutron cross section of silver (see Fig. 2), makes this element useful as a thermal neutron flux monitor for reactor surveillance programs (see Nuclearreactors). 

From the 1960s onward, there was a greater interest in psychological issues, dominated by the concept of the human as a single-channel processor of information. This stimulated research into a number of areas. Studies of mental workload were concerned with the ability of humans to cope with extremely high levels of information in situations such as air traffic control. Vigilance studies, which focused on the human s role in situations with very low levels of stimulation such as radar monitoring, represented the other extreme of human performance that was considered. 

The biological activity of the polymers was evaluated in vitro by the ability to stimulate the release of superoxide from DMSO-differentiated HL-60 cells [18-20]. The released superoxide was monitored by the cytochrome C method [21,22]. As shown in Figure 7, when the differen-... 

The majority of functional assays involve primary signaling. In the case of GPCRs, this involves activation of G-proteins. However, receptors have other behaviors— some of which can be monitored to detect ligand activity. For example, upon stimulation many receptors are desensitized through phosphorylation and subsequently taken into the cell and either recycled back to the cell surface or digested. This process can be monitored by observing ligand-mediated receptor internalization. For... 

Respiratory depression can be a serious event requiring administration of a respiratory stimulant. When an analeptic is administered, the nurse notes and records the rate, depth, and character of the respirations before the drug is given to provide a database for evaluation of the effectiveness of drug therapy. Oxygen is usually ordered for before and after administration of a respiratory stimulant. After administration, the nurse monitors respirations closely and records the effects of therapy. 

Older adults are especially sensitive to the effects of the CNS stimulants and may exhibit excessive anxiety, nervousness insomnia, and mental confusion. Cardiovascular disorders common in the older adult, maybe worsened by the CNS stimulants Careful monitoring is important because the presence of these reactions may result in the need to discontinue use of the drug. 

FIGURE 4-23 Experimental setup for monitoring dopamine release by exocytosis, from a cell body. The microelectrode and glass capillary (containing the chemical stimulant) are micromanipulated up to the cell body. (Reproduced with permission from reference 82.)... 

Succinylcholine-induced rhabdomyolysis Hypoxic encephalopathy Intrathecal administration of constant agents Defective temperature-monitoring devices Stimulation during light anesthesia... 

To ensure that the inhibition of EGF binding by palytoxin was not a consequence of cell toxicity, the effect of palytoxin on DNA synthesis in Swiss 3T3 cells was monitored. When cells were incubated in the presence of palytoxin, 10% fetal calf serum, and H-thymidine for 19.5 hr, no depression in the extent of H-thymidine incorporation into DNA was detected up to 3.7 pM palytoxin (Table I). Although 11 pM palytoxin was toxic when present for a prolonged period, under the conditions of the assays described above no toxicity was detected (Table I). When cells were incubated in the presence of palytoxin, 0.1% fetal calf serum, and H-thymidine, palytoxin did not stimulate significant incorporation of H-thymidine into DNA. Thus, although it can modulate the EGF receptor system under these conditions, palytoxin alone does not appear to be mitogenic for Swiss 3T3 cells. 

Figure 1.9 Comparison of the effects of an endogenously released and exogenously applied neurotransmitter on neuronal activity (identity of action). Recordings are made either of neuronal firing (extracellularly, A) or of membrane potential (intracellularly, B). The proposed transmitter is applied by iontophoresis, although in a brain slice preparation it can be added to the bathing medium. In this instance the applied neurotransmitter produces an inhibition, like that of nerve stimulation, as monitored by both recordings and both are affected similarly by the antagonist. The applied neurotransmitter thus behaves like and is probably identical to that released from the nerve...

One approach, and the first to be adopted, is to study transmitter release from slices which have been preloaded with radiolabelled transmitter. In these experiments, drug-induced changes in the release of transmitter is usually monitored using the doublepulse technique. This involves comparing the effects of a test drug on the amount of transmitter released in response to a reference pulse and a second identical test pulse. If all the radiolabelled transmitter that overflows in the effluent is collected, and the amount which remains in the slice at the end of the experiment is also measured, it is possible to calculate not only how much radiolabelled transmitter was originally contained in the slice but also the effects of drugs on fractional release , i.e. the proportion of the store of radiolabelled transmitter which is released by nerve stimulation. As with... 

However, it has been established that an intense control of certain variables may improve the execution of a hydraulic fracturing job and the success of a stimulation. Therefore an intense quality control is recommended [552,553]. Such a program includes monitoring the breaker performance at low temperatures and measuring the sensitivity of fracturing fluids to variations in crosslinker loading, temperature stabilizers, and other additives at higher temperatures. 

Measurements of individual laboratory performance provides for comparisons between laboratories. It then follows to ask why some laboratories report data that are more accurate and precise than do their peers, and a well designed external quality assessment scheme allows investigation of some of the important factors (see below). A comparison of performance between individual laboratories also helps to stimulate those who are not so successful to improve (or abandon the assay) and those who do well to continue with their expertise. Finally, changes of performance may be monitored as a consequence of some new factor, e.g. purchase of a new piece of equipment, work carried out by a different analyst, change to the methodology etc. 

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