Staphylococcus aureus protein A

Steidler L, Remaut E, Fiers W, LamB as a carrier molecule for the functional exposition of IgG-binding domains of the Staphylococcus aureus Protein A at the surface of Escherichia coli K12, Mot. Gen. Genet., 236 187-192, 1993. 

Djojonegoro BM, Benedik MJ, Willson RC, Bacteriophage surface display of an immunoglobulin-binding domain of Staphylococcus aureus protein A, BioTechnology, 12 169-172, 1994. 

When the IgG is of a subclass whose Fc binds to Staphylococcus aureus protein A, " an affinity column of insolubilized protein A can be used to remove Fc-containing fragments as follows. 

Graille M, Stura EA, Corper AL, Sutton BJ, Taussig MJ, Charbonnier JB, Silverman GJ Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody structural basis for recognition of B-cell receptors and superantigen activity. Proc Natl Acad Sci USA 2000 97 5399-5404. 

Antibody fragment libraries from immunized and nonimmunized sources can be used in phage display and peptide libraries are commercially available. An alternative technique is to design protein aptamers that consist of a stable protein scaffold on which random peptides are displayed. An example of protein aptamers are affibodies, which present a library of 13 randomized amino acids on the Z domain of Staphylococcus aureus protein A. Crystal structure studies indicate similarity in the binding of an affibody to its target to protein-antibody interactions. However, affibodies have a dissociation constant of approximately 1 /.tM compared to antibody-antigen complexes ofl nM orless(H3, Rl, Wl). 

Mineral oil [liquid paraffin) was also used for the formation of pre-polymerization droplets [Kempe and Kempe, 2006). Being chemically inert in nature, these liquids do not affect the non-covalent interactions in template-monomer complex when used as a dispersing medium. There are some recent reports utilizing suspension polymerization for MIP synthesis for Staphylococcus aureus protein A-imprinted polyacrylamide [Pan et al., 2009), Stlgmasterol MIP microspheres [Han et al., 2008), MIP hydrogels for the peptide hepcidin [Abbate et al., 2010) and Promethazine based MIPs [Alizadeh et al., 2012). 

Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene... 

Gomez MI, Lee A, Reddy B et al. Staphylococcus aureus protein A induces airway epithelial inflammatory responses by activating TNFRl. Nat Med 2004 10 842-848. 

Staphylococcus aureus (protein A-bearing) He,E,S ALP/NADP/ADH/ diaphorase Pt electrode lOpgmL protein A 92... 

Gomez, M. I., Lee, A., Reddy, B., Muir, A., Soong, C., Pitt, A., Cheung, A. and Prince, A. Staphylococcus aureus protein A induces airway epithelial inflammatory responses by activating TNFRl. Nat. Med. 10 842-848, 2004. 

Hoffman, W. L., Ru es, A. O. and Tabarya, D. Chicken anti-protein A prevents Staphylococcus aureus protein A from binding to human and rabbit IgC in immunoassays and eliminates most false positive results. /. Immunol. Methods 198 67-77, 1996. 

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