Stable Isotope Probes

This procedure makes it possible to relate function in the environment to specific organisms or enzymes that carry out degradation. 

A phenol-degrading community was examined using phenol followed by analysis of the stable-isotope-labeled RNA by equilibrium density centrifugation, and complemented 

The population of putative methanotrophs in a peat soil microcosm was examined by 

It has been established that methane is produced on rice roots by reduction of CO2. This was examined in rice roots using a combination of 16S rRNA sequencing and density gradient fractionation of C-labeled DNA after incubation with C02. The major groups of archaea detected were Methanosarcinaceae that decreased with time to be replaced by the hitherto uncultured Rice Cluster I, although the former subsequently dominated (Lu et al. 2005). 

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Lueders T, B Pommerenke, MW Friedrich (2004) Stable-isotope probing of microorganisms thriving at thermodynamic limits syntrophic propionate oxidation in flooded soil. Appl Environ Microbiol 70 5778-5786. 

Lu Y, T Lueders, MW Friedrich, R Conrad (2005) Detecting active methanogenic populations on rice roots using stable isotope probing. Environ Microbiol 1 326-336. 

DeRito CM, GM Pnmphrey, EL Madsen (2005) Use of field-based stable isotope probing to identify adapted popnlations and track carbon flow throngh a phenol-degrading soil microbial conunnnity. Appl Environ Microbiol 71 7858-7865. 

Kasai Y, Y Takahata, M Manefield, K Watanabe (2006) RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater. Appl Environ Microbiol 72 3586-3592. 

Lueders T, B Wagner, P Claus, MW Friedrich (2004a) Stable isotope probing of rRNA and DNA reveals a dynamic methylotroph community and trophic interactions with fungi and protozoa in oxic rice field soil. Environ Microbiol 6 60-72. 

Manefield M, AS Whiteley, RI Griffiths, MJ Bailey (2002) RNA stable isotope probing, a novel means of linking microbial community function to phytogeny. Appl Environ Microbiol 68 5367-5373. 

Morris SA, S Radajewski, TW Willison, JC Murrell (2002) Identification of the functionally active methano-troph population in a peat soil microcosm by stable-isotope probing. Appl Environ Microbiol 68 1446-1453. 

Radajewski S, G Webster, DS Reay, SA Morris, P Ineson, DB Nedwell, 11 Prosser, JC Murrell (2002) Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing. Microbiology (UK) 148 2331-2341. 

Wellington, E. M. H. Berry, A. Krsek, M. Resolving functional diversity in relation to microbial community structure in soil Exploiting genomics and stable isotope probing. Curr. Opin. Microbiol. 2003, 6,295-301. 

Lu YH, Conrad R. In situ stable isotope probing of methanogenic archaea in the rice rhizosphere. Science. 2005 309 1088-1090. 

Radajewski, S., Ineson, P., Parekh, N. R. Murrell, J. C. (2000). Stable-isotope probing as a tool in microbial ecology. Nature, 403, 646-9. 

More novel insights into marine nitrogen may be just around the comer with the influx of new and improved methodologies and analytical capabdities. Stable isotope probing has been used with enriched C isotopes to identify directly active... 

Buckley, D. H., Huangyutitham, V., Hsu, S. F., and Nelson, T. A. (2007). Stable isotope probing with N-15(2) reveals novel noncultivated diazotrophs in soil. Appl. Environ. Microbiol 73, 3196—3204. 

Friedrich, M. W. (2006). Stable-isotope probing of DNA insights into the function of imcultivated microorganisms from isotopicaUy labeled metagenomes. Curr. Opin. Biotechnol. 17, 59—66. 

Manefield, M., Whiteley, A. S., Osde, N., Ineson, P., and Bailey, M.J. (2002b). Technical considerations for RNA-based stable isotope probing An approach to associating microbial diversity with microbial community fimction. Rapid Commun. Mass Spectrom. 16, 2179—2183. 

Cadish, G., Espana, M., Causey, R., Richter, M., Shaw, E., Morgan, J. A. W., Rahn, C., and Bending, G. D. (2005). Technical considerations for the use of N-DNA stable-isotope probing for functional microbial activity in sods. Rapid Commun. Mass Spectrom. 19, 1424-1428. 

Hutchens, E., Radajewski, S., Dumont, M. G., McDonald, I. R., and Murrell, J. C. (2004). Analysis of methanotrophic bacteria in Movde Cave by stable isotope probing. Environ. Microbiol. 6, 111-120. 

Kreuzer-Martin, H. W. 2007. Stable isotope probing linking functional activity to specific members of microbial communities. Soil Sci. Soc. Am. J. 71 611-619. 

Ln, Y. H., W. R. Abraham, and R. Conrad. 2007. Spatial variation of active microbiota in the rice rhizosphere revealed by in sitn stable isotope probing of phospholipid fatty acids. Environ. Microbiol. 9 474-481. 

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