Stable isotope dilution analysis

Stable-isotope dilution analysis is an analytical technique in which a known quantity of a stable-labelled isotope is added to a sample prior to extraction, in order to quantitate a particular compound. The ratio of the naturally abundant and the stable-labelled isotope is a measure of the naturally abundant compound and can be determined only by gas chromatography-mass spectrometry since the naturally abundant and the stable-labelled isotope cannot be completely separated gas chromatographically. 

Diagnosis of medium chain acyl-CoA dehydrogenase deficiency by stable isotope dilution analysis of urinary acylglycines retrospective and prospective studies, and comparison of its accuracy to acylcamitine identification by FAB/mass spectrometry. 

Kok RM, Howells DW, van den Heuvel CC, Guerand WS, Thompson GN, Jakobs C (1993) Stable isotope dilution analysis of GABA in CSF using solvent extraction and electron-capture negative-ion mass fragmentography. J Inherit Metab Dis 16 508-512... 

Stellaard F, ten Brink HJ, Kok RM, van den Heuvel L, Jakobs C (1990) Stable isotope dilution analysis of very long chain fatty acids in plasma, urine and amniotic fluid by electron capture negative ion mass fragmentography. Clin Chim Acta 192 33-144... 

Vreken P, Van Lint AEM, Bootsma AH, Overmars H, Wanders RJA, Van Gennip AH (1998) Rapid stable isotope dilution analysis of very-long-chain fatty acids, pristanic acid and phytanic acid using gas chromatography-electron impact mass spectrometry. J Chromatogr 713 281-287... 

Certain techniques such as neutron activation analysis and stable isotope dilution analysis offer very powerful and sensitive methods for the determination of trace metals, but involve considerable effort and access to extensive facilities and so are not suited for routine laboratory determinations. However, they have been used to determine trace metal levels accurately in homogeneous biological materials, which are now used as reference materials for other techniques. 

G. S. Schuhmann and J.A. Philpotts, Mass-spectrometric stable-isotope dilution analysis for lanthanides in geochemical materials 471... 

Comparative aroma dilution analyses of the headspace of aqueous solutions, containing either the total volatiles isolated from a fresh coffee brew or these volatiles mixed with the melanoidins isolated from coffee brew, revealed drastic losses of odorous thiols, 2-furfurylthiol, 3-methyl-2-butenethiol, 3-mercapto-3-methylbutyl formate, 2-methyl-3-furanthiol, and methanethiol, in the presence of melanoidins.509 The first compound was affected most, the reduction being 16-fold, and was accompanied by an overall reduction in roasty-sulfury aroma. The rapid loss of thiols was confirmed by stable-isotope dilution analysis. [2H]-NMR and LC-MS gave strong evidence that the thiols become covalently bound via Maillard-derived pyrazinium compounds. 

P. Schieberle, Quantitation of important roast-smelling odorants in popcorn by stable isotope dilution analysis and model studies on flavor formation during popping, J. Agric. Food Chem., 1995, 43, 2442-2448. 

Jakobs C, Dorland L, Wikkerink B, Kok RM, de Jong AP, Wadman SK. Stable isotope dilution analysis of succinyiacetone using electron capture negative ion mass fragmentography an accurate approach to the pre- and neonatal diagnosis of hereditary tyrosinemia type I. CHn Chim Acta 1988 171 223-31. 

Stable isotope dilution analysis of hydrologic samples by ICP-MS, Anal Chem 59 1568-1575. 

Garbarino, J.R. and Taylor, H.E., Stable-isotope-dilution analysis of hydrologic samples by inductively coupled plasma mass spectrometry. Anal. Chem., 59, 1568-1575 (1987). 

Hasegawa, H. Matsukawa, T. Shinohara, Y. Hashimoto, T. Assessment of the metabohc chiral inversion of D-leucine in rat by gas chromatography-mass spectrometry combined with a stable isotope dilution analysis. Drug Metab. Disp. 2000, 28, 920-924. 

Rinaldo, R, O Shea, J.J., Welch, R.D., Tanaka, K. (1990) Diagnosis of medium chain acyl-CoA dehydrogenase deficiency by stable isotope dilution analysis of urinary acyl-glycines retrospective and prospective studies, and comparison of its accuracy to acylcarnitine identification by FAB/mass spectrometry. Prog. Clin. Biol. Res., 321, 411-418. 

Dahmen, L, Pfluger, M., Martin, M., Rottmann, L., Weichbrodt, G. (1997) Trace element determination of high-purity chemicals for the processing of semiconductors with high-resolution ICP-mass spectrometry using stable isotope dilution analysis (IDA). Fresenius Journal of Analytical Chemistry, 359,410-413. 

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