SPR sensor chips

Fig. 13a-e. The increase of the signal intensities by the addition of the dendritic complexes composed of IgGs and protein A. The hapten was immobilized to the surface of the SPR sensor chip. The increase of the signal intensities on the complex formation of hapten with the antibodies were monitored. The addition of mouse IgG specific for hapten (Abl) (a), the complex of the Abl with protein A (b), one to one complex of Abl with anti-mouse IgG (Fc) antibody (Ab2) (c), two to one complex of Abl with Ab2 (d), and two to one complex of Abl with Ab2 in the presence of protein A (e)... 

Fig. 12 (a) Schematic representation of the preparation of a molecularly imprinted polymer with immobilized Au nanoparticle and the detection of an analyte upon selective swelling of the MIP. Reprinted with permission from [123]. Copyright (2004) American Chemical Society, (b) Schematic representation of Au-MIP/MIP-coated SPR sensor chip. Reprinted with permission from [124]. Copyright (2005) American Chemical Society... 

Detection of phenols based on toxicity measurement was carried out by Chio et al. [40]. In their experiments, they used a Multiscope SPR sensor (Optrel, Germany) and Escherichia coli cells immobilized on an SPR sensor chip via synthetic cystein-terminated oligopeptides. When the immobilized cells were exposed to phenol, a decrease in the SPR signal was observed due... 

An assay for diagnosing type I diabetes mellitus based on the detection of anti-glutamic acid decarboxylase (GAD) antibodies in buffer by a Biacore 2000, is presented in [47,48]. Biotinylated GAD was immobihzed on a streptavidin-coated surface. The effect of mixed SAM composition (differing in ratios of hydroxyl- and carboxyl-terminated alkanethiols) on the sensitivity of the sensor was investigated. On SPR sensor chips prepared with the optimized SAM composition (10 1 ratio of 3-mercaptopropanol to 11-mercaptoundecanoic acid), a concentration of anti-GAD as low as... 

Theophylline-imprinted polymers prepared with MAA and EDMA were utilized for SPR sensors, in which the particles were immobilized on the silver film on the SPR sensor chip by evaporation from acetonitrile-acetic acid (99 1 v/v) containing the particles [20]. The detection limit was reported to be 0.4 mg/mL of theophylline in aqueous solution. 

Matsui, J., Akamatsu,K., et al. SPR Sensor Chip for Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticles. "Analytical Chemis-try,77(13), 4282 285 (2005). 

Glycolipid 1 and matrix lipid 2 at various concentrations (1 / 2 = 1 / 0, 5 / 1, 9/1,19/1 and 0 / 1) in chloroform (lO MlO M) was spread on the surface of an aqueous subphase. After waiting for 10 min to vaporize the chloroform, the mixed lipids were compressed to the pressure of 30 mN/m at a rate of 0.1 nm /molecule/min to form a monolayer. The monolayer was equilibrated for 10 min under the same surface pressure and polymerized by UV irradiation (254 nm, 8 W) for 10 min. The distance between the lamp and the monolayer surface was 12 cm. The polymerized monolayer was transferred onto the OTS (octadecyltrichlorosilane) coated glass plate or the SPR sensor chip carrying... 

Monitoring the substrate binding to the MBP-GalT array on SPR sensor chip... 

The sensitivity of SPR biosensors was also improved by increasing the amount of ligand biomolecules attached on the SPR sensor chips. As mentioned above, the ligand... 

Y. Kuriu, A. Kawamura, T. Uragami, T. Miyata, Pormation of thin molecularly imprinted hydrogel layers with lectin recognition sites on SPR sensor chips by atom transfer radical polymerization, Chem. Lett. 43 (2014) 825-827. 

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