Spectrum of sucrose

Fig. 4. (a) Conventional 500 MHz HMBC spectrum of sucrose octaacetate in deuteriochlo-roform, and (b) HMBC spectrum with ti-noise corrected by reference deconvolution. (Reproduced, with permission, from Signal Treatment and Signal Analysis in NMR, ed. D.N. Rutledge, Elsevier, Amsterdam, 1996)... 

The 3700-2700-cm-1 region is less easy to interpret than the region below 1700 cm-1. Nevertheless, comparative study of intensities of the C-H vibrations permitted differentiation of D-fructose from D-glucose and sucrose. It was found187 that the asymmetrical vibration pas(C-H) for CH2 in d-fructose is stronger than the symmetrical vibration the opposite was observed for D-glucose, and the spectrum of sucrose exhibits almost the same intensity for the two vibrations. This result (see Fig. 19), which could... 

Bands Observed" in the Laser-Raman Spectrum of Sucrose in Aqueous Solution... 

Fig. 18. Substrate spectrum of sucrose synthase (EC 2.4.1.13) from rice grains in the cleavage reaction of sucrose (24) with nucleoside diphosphates [272,273]...

A planar microcoil-based probe was fabricated on a glass chip for NMR measurement (see Figure 7.47). The H NMR spectrum of sucrose in D20 was shown in Figure 7.48. The noise is mainly due to the thermal fluctuations generated by the series resistance of the coil (e.g., 1 2). To reduce the channel wall thickness... 

FIGURE 7.49 H NMR spectrum of sucrose after Lorentz-Gauss resolution enhancement applied to the data shown on Figure 7.48b [797]. Reprinted with permission from Elsevier Science. 

Figure 1. Triboluminescence Spectrum of Sucrose. Spectrum obtained with 5, 10 sec acquisitions and a 25 /im slit using a 142X chevron detector.

Figure 8.15(b) shows the spectrum of sucrose with a 90° Gaussian pulse applied to the triplet at 3.99 ppm, compared to a normal 1H spectrum (Fig. 8.15(a)). This is done by moving the reference frequency to place the 3.99 ppm triplet at the center of the spectral window (on-resonance), which is the center of the Gaussian-shaped excitation profile... 

Figure 9.28 shows the 300 MHz DQF-COSY spectrum of sucrose in D2O. For convenience, the H spectrum is shown at the top and on the left side. To analyze the COSY spectrum you always need a starting point a H resonance that is resolved and can be... 

Figure 11.3 shows the downfield portion of the spectrum of sucrose (gl doublet, /HH = 3.8 Hz) with normal vertical scaling and with the vertical scale increased by a factor of 100 to show the 13C satellites. The satellites show the same doublet 7rh coupling observed in the 12C-bound proton signal (7=3.8 Hz), with an additional 169.6 Hz coupling to the 13C nucleus (Vch)- The peak height is roughly half (actually half of 1% because the vertical scale is 100 x) of the central peak because they are part of a doublet with concentration about 1% of the concentration of the 12C species. If you look closely you will see that the center of the double doublet is not exactly the same chemical shift... 

Figure 10 (A) H NMR spectrum of the trace impurity sample (200 pM atenolol and 200 mM sucrose in 50% TE/D20) from 5-mm probe. The expanded and vertically increased area is shown. Microcoil H NMR spectra shown in (B)-(D) recorded and processed with identical parameters. (B) Static NMR spectrum obtained with direct injection of 25 mM atenolol to the NMR microcoil. S/N of atenolol methyl peak is 21. (C) On-flow cITP-NMR spectrum of atenolol sample band at peak maximum during analysis of the trace impurity sample (200 pM atenolol and 200 mM sucrose in 50% TE/D20). No sucrose peaks can be observed. S/N atenolol methyl peak is 34. (D) Stopped-flow cITP-NMR spectrum of sucrose at peak maximum from the same experiment as in (C). (Adopted with the permission from Ref. 41. Copyright 1998 American Chemical Society.)...

Note that the information provided here is similar to that found in the INADEQUATE spectrum of sucrose (Fig. 12.4ft), but the sensitivity ofTOCSY is much greater. The upper 2D spectrum shows a TOCSY spectrum with a short contact time, 10 ms, where the cross peaks are restricted largely to protons that are directly coupled (as in COSY). Courtesy of Daron Freedberg (Food and Drug Administration). 

FIG. 8 C nmr spectrum of sucrose (22.6 MHz). Not all lines have been assigned to individual carbon atoms. 

Fig. 8.—The Partial, H Nuclear Magnetic Resonance Spectrum of Sucrose Octaacetate (8) in Solution in Acetone-rid, With a Diagrammatic Representation of the First-order Assignment of the n-Glucose Protons is Shown in A. [The set of INDOR traces (B, C, D, and E) was obtained by sequentially monitoring transitions 1, 8, 5, and 3.]...

Figure 3.25 Homonuclear decoupling experiments of the 300 MHz proton NMR spectrum of sucrose dissolved in D2O. The fully coupled spectrum is shown in (c). (a) Selective saturation of the triplet at 4.05 ppm collapses the doublet at 4.22 ppm, showing the coupling between the positions of protons a and b marked on the sucrose structure, (b) Saturation of the doublet at 5.41 ppm collapses the doublet of doublets at 3.55 ppm, leaving a doublet. The experiment shows the coupling between the protons marked c and d on the structure. (The spectra are from Petersheim, used with permission. The sucrose structure is that of D-(- -)-sucrose, obtained from the SDBS database, courtesy of National Institute of Industrial Science and Technology, Japan, SDBSWeb http //www. aist.go.jp/RIOBD/SDBS. Accessed 11/05/02.)...

Figure 3.40 Heteronuclear decoupling of the 75 MHz spectrum of sucrose. The fully coupled spectrum is at the top. The bottom spectrum is the broadband decoupled spectrum. The structure of sucrose was given in Fig. 3.25. The molecule contains 12 nonequivalent carbon atoms the decoupled spectrum clearly shows 12 single peaks, one for each nonequivalent C atom. (From Petersheim, used with permission.)...

Figure 3.51 A 2D-HETCOR experiment, (a) 75 MHz spectrum of 1 M sucrose in D2O. (b) 300 MHz H spectrum of 1 M sucrose in D2O. In both spectra the labels G and F refer to the glucose ring and the fructose ring, respectively. Structure of sucrose was given in Fig. 3.25. (c) The 2D-HETCOR spectrum of sucrose. Carbon F2 has no protons directly bonded to it because there is no spot of intensity in the HETCOR plot in line with the F2 chemical shift.

The spectrum of sucrose in aqueous solution has been studied to examine further the potential of F.t.i.r. spectroscopy for determining carbohydrate configurations. While bands characteristic of an a- rather than 6-linked D-glucopyranosyl unit were present, no criteria for discerning the stereochemistry of the fructose unit were found. Some bands in the hydroxyl stretching region of crystalline carbohydrates have been identified through a... 

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