Solid phase extraction vacuum manifold

Solid-phase extraction vacuum manifolds for simultaneous processing of 10, 12, 20, and 24 SPE tubes. 

Figure 12 Typical shallow-well 96-well format solid-phase extraction vacuum manifold adapted for use with liquid-handling workstations.

Products/technologies These include Visiprep solid phase extraction vacuum manifolds to manipulate combinatorial chemistry reaction mixtures a rare chemicals library of over 60,000 products CombiKits to create a customized kit of preweighed building blocks SUPELCOSIL ABZ+ HPLC columns and a chemical directory on CD-ROM. 

Vacuum manifold for multiple solid phase extraction. 

Following this step, solid phase extraction (SPE) was performed on all samples, using Oasis MCX cartridges (lcc/30 mg from Waters), a vacuum manifold device and a vacuum source (pump)... 

Vacuum manifold and accessories Applications Guide, QA/QC Reports Solid-phase extraction products... 

Figure 8.6 Schematic of a commercially available vacuum manifold system used in solid-phase extraction.

Figure 12-9. Vacuum manifolds for solid phase extraction. Left, 12 port right, 24 port.

FIGURE 3. Schematic diagram showing the typical construction of a solid-phase extraction cartridge and a vacuum manifold for parallel sample processing. 

FIQ. IB.S. 16-Port vacuum manifold for use with solid-phase extraction tubes. (Courtesy of Alltech.)... 

Vacmaster vacuum manifold to conduct solid-phase extraction (SPE) from International Sorbent Technology the manifold enables 10 extractions to be performed at the same time Water trap and associated vacuum tubing to be used with the Vacmaster... 

Solid-phase extraction (SPE) offers an alternative way for enrichment of chlorinated fatty acids. Akesson-Nilsson presented an aminopropyl-based SPE technique for enrichment of chlorinated FAMEs in a cell-culture medium and for previously silver nitrate and urea treated eel lipids. In this application, a 500-mg aminopropyl column was connected to a vacuum manifold and conditioned with 2 ml of hexane. After transesterified lipid samples in 0.2 ml of hexane were loaded, the column was first eluted with 6 ml of hexane and then with 4 ml of a solvent mixmre made of hexane-diethyl ether-dichloromethane (89 1 10). In this way, the majority of non-chlorinated FAMEs were removed in the first elution and chlorinated FAMEs enriched in the later elution. 

Solid-Phase Extraction (SPE) SPE generally provides the cleanest sample extract among all sample preparation techniques in terms of selectivity. SPE uses the affinity of analytes in a liquid phase for a stationary phase, through which the samples pass with the analytes of interest retained on the stationary phase. The unwanted portion that passes through the stationary phase is discarded. The analytes retained on the stationary phase can then be removed for collection. The stationary phase is normally in the form of a packed column, a packed cartridge, or a 96-well plate [4,29], each of which can be mounted on a specific type of extraction manifold. The manifold allows multiple samples to be processed simultaneously. AppUcation of vacuum (negative mode) or pressure (positive mode)... 

Solid-phase extraction (SPE micro-columns) was efficient for the purification of alkaloidous solutions. Aliquots were passed through a pre-activated (by methanol than water) SPE cartridge (Supelclean LC-8 columns 500 mg, 3 mL, supplied by Supelco) which was then washed with water. Extraction was performed on a vacuum manifold processor (LiChrolut extraction unit Merck).The alkaloid (lobeline)-containing fraction was eluted from the tube with methanol. On the basis of the HPLC determination, the recovery of lobeline from the SPE step was total [40]. 

Solid-phase extraction is a relatively new sample preparation method based on chemically modified silica gel or other sorbent type packed into a small plastic disposable cartridge or column. The cartridges are usually coimected to the end of a syringe and samples passed through by pressure, and the columns and disks are normally used in a vacuum manifold. Sorbents with various functional groups can be obtained in different configurations and sizes from commercial suppliers, which can supply procedures for analyses and bibliographies of applications. 

FIGURE 15.9 Diagram of a vacuum manifold used for solid-phase extraction (SPE) of... 

A typical solid phase extraction manifold. The cartridges drip into the chamber below, where tubes collect the effluent. A vacuum port with gauge is used to control the vacuum applied to the chamber. 

All methods for the generation of ions for mass spectrometry described up to this point require the analyte for ionization to be presented either directly under high vacuum (El, Cl, FI, FD) or contained in a sort of solution from which ions are to be extracted into or generated in the gas phase (FAB, LDI, MALDI). Even the atmospheric pressure ionization techniques employ processes that create ions from dilute (solid) solutions of the sample (ESI, APCI, APPI, AP-MALDI). This chapter deals with the manifold methods and interfaces which are allowing to overcome these limitations, and which have developed at a breathtaking pace within the short time since the publication of the first edition of this book. 

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