Solanum tuberosum’, lectin

Gabor, F., Wirth, M., Jurkovich, B., Theyer, G., Walcher, G., Hamilton, G., Lectin-mediated bioadhesion Proteolytic stability and binding-characteristics of wheat germ agglutinin and Solanum tuberosum lectin on Caco-2, HT-29 and human colonocytes. J Control Release 49, 27-37 (1997). 

Solanum tuberosum lectin Solanum tuberosum (potato) (GlcNAc)3... 

For the modification of the gold surface different chemical and biological substances (dodecanethiol, lectins, dextran sulfate DS, with the mass of 5 kDa and polyelectrolytes) were used. The mercaptane layer was formed in ethanol atlO" M concentration of thiol. Polyaniline hydrochloride (PAH), Aldrich , USA, was used to cover the transducer surface by a water insoluble polymer. Lectins from Phaseolus vulgaris (PLA), Solanum tuberosum (STA), Helix pomatia (HPA) and Tuberosum vulgaris (WGA) were immobilized on the surface pre-treated with dodecanethiol or PAH. These lectins were used for the immobilization of glycoproteins. Blocking of the free binding sites was carried out with 1% bovine serum albumin (BSA). 

Although seeds are the common source of lectin activity, there are reports (some contradictory) of agglutinins occurring in leaves, stems, and roots.77,94 For example, black-locust (Robinia pseudoaccacia) lectin160 was isolated from its bark, potato (Solanum tuberosum) lectin161 from its tubers, and poke-weed (Phytolacca americanum) mitogen162 from its roots. A new class of plant cell-wall and membrane-bound lectins ( /3-lectins ) has been described.116,163,164 A treatise collected several schemes for the purification of lectins.165,1658... 

Fig. 15.—Human IgA, Glycopeptide871 Showing the Carbohydrate-binding Loci for Various Lectins, [a. Limulus polyphemus b, Triticum vulgaris c, Ricinus communis d, Sophora japonica e, Abrus precatorius f, Cytisus sessilifolius g, Phaseolus vulgaris-, h, Canavalia ensiformis i, Lens culinaris j, Pisum sativus k, Vicia faba-, 1, Bandeiraea simplicifolia II m, Solanum tuberosum and n, Ulex europeus II.]...

The lectin from potato, Solanum tuberosum, has been purified by affinity chromatography on an immobilized AW iV -triacetyl-chitotriose matrix,... 

The extensins are highly insoluble components of cell walls of terrestrial plants and are considered to be important in the plastic extension of the typical cell wall of the land plants (Lamport, 1970, 1973). However, some of the related arabinoproteins are found elsewhere in the cell, and one of the best studied is the readily soluble lectin of the potato, Solanum tuberosum (Allen and Neuberger, 1973), which has a high content of carbohydrate. 

Ashford, D., Desai, N.N., Allen, A.K., Neuberger, A., O Neill, M.A. Selvendran, R.R. (1982) Structural Studies of the Carbohydrate Moieties of Lectins from Potato (Solanum Tuberosum) Tubers and Thorn-apple ( Datura stramonium) Seeds , Biochemical Journal, 201, 199-208... 

Affinity chromatography of mammalian phospho-fructokinase on immobilized adenine nucleotides Studies of the effect of the chain-length on the adsorption of albumin removal of albumin from sera Purification of commercial preparations of urokinase Purification of Solanum tuberosum agglutinin (sta-lectin)... 

Purification of Solanum tuberosum agglutinin (sta-lectin) Competitive affinity chromatography of glycoside hydrolases from soybeans and in Takadiastase preparations... 

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