Sodium bicarbonate , buffer

Aldehydes can be dkeedy converted to esters using bromine in alcohol solvents with sodium bicarbonate buffer (41). 

Dissolve a first protein to be modified at a concentration of l-10mg/ml in 0.1 M sodium bicarbonate buffer, pH 8.5. PBS buffer at physiological pH also may be used as the reaction medium. 

Chemical/Physical. Endosulfan detected in Little Miami River, OH was readily hydrolyzed and tentatively identified as endosulfan diol (Eichelberger and Lichtenberg, 1971). Undergoes slow hydrolysis forming the endosulfan diol and sulfur dioxide (Worthing and Hance, 1991). The hydrolysis half-lives at pH values (temperature) of 3.32 (87.0 °C), 6.89 (68.0 °C), and 8.69 (38.0 °C) were calculated to be 2.7, 0.07, and 0.04 d, respectively (Ellington et al., 1988). Greve and Wit (1971) reported hydrolysis half-lives of P-endosulfan at 20 °C and pH values of 7 and 5.5 were 37 and 187 d, respectively. In a 1 pM sodium bicarbonate buffer solution at pH 8.15 and 28 °C, suspensions of sea sand, titanium dioxide, a-ferric oxide, a-FeOOH, laponite, and silicon dioxide catalyzed the hydrolysis of a-endosulfan to endosulfan diol. The uncatalyzed hydrolysis rate constant and half-life was 4.01 x lO Vsec and 0.20 d, respectively (Walse et al., 2002). 

Azo and azoxy compounds are potential by-products of peroxyacetic acid oxidations, particularly when the rate of oxidation is slow. Competitive condensation reactions are usually avoided by using an excess of oxidant and also avoiding the presence of excess acid which retards amine oxidation. The latter is sometimes suppressed by using a sodium bicarbonate buffer.i i i ... 

It is possible to perform an oxidation with Dess-Martin periodinane under almost neutral conditions by adding pyridine to the reaction flask in order to neutralize the acetic acid, which is generated during the oxidation, and performing the work-up by treatment with sodium thiosulfate in the presence of a sodium bicarbonate buffer.2... 

Epoxidation of aromatic hydrocarbons is an important method for the preparation of arene oxides. m-Chloroperbenzoic acid (MCPBA) is used in a two-phase system that involves treating the hydrocarbon with a large excess ( 10-fold) of MCPBA in methylene chloride-aqueous sodium bicarbonate at room temperature. The yields are moderate (10-60%). Because the arene oxides are sensitive to acids, the presence of sodium bicarbonate buffer is necessary. A number of K-region (see Section VII for a definition) epoxides like phenanthrene 9,10-oxide (1, 59%), 9,10-dimethylphenanthrene 9,10-oxide (2,40%), 9-phenylphenanthrene 9,10-epoxide (3,50%), pyrene 4,5-oxide (4, 14%), and chrysene 4,5-oxide (5,9%) have been prepared by this method.9... 

Antibody-coupling buffer (0.1M sodium bicarbonate buffer, pH 8.3). 

Vinyl acetate-butyl acrylate copolymers (0-100% butyl acrylate) were prepared by both batch and starved semi-continuous polymerization using sodium lauryl sulfate emulsifier, potassium persulfate initiator, and sodium bicarbonate buffer. This copolymer system was selected, not only because of its industrial importance, but also because of its copolymerization reactivity ratios, which predict a critical dependence of copolymer compositional distribution on the technique of polymerization. The butyl acrylate is so much more reactive than the vinyl acetate that batch polymerization of any monomer ratio would be expected to give a butyl acrylate-rich copolymer until the butyl acrylate is exhausted and polyvinyl acetate thereafter. 

Air (chromium(VI)) Sample collected in filters containing sodium bicarbonate buffer at 15 L/minute Ion chromatography/ coulometric 0.01 ng/m3for 20 m3 sample 94% Sheehan et al. 1992... 

Sodium bicarbonate Buffer agent, isotonicity, pH adjustment, solubilizer, stabilizer iv, im, sc, is, ic, other inj. 

Re-Al-acetylation of the amino sugars using acetic anhydride in aqueous sodium bicarbonate buffer. 

A monoclonal anti-digoxin antibody (mouse) used for the ELISA was produced and prepared by Sawada et al. [121]. An ELISA plate (96 well) was coated with ca. 100 ng/well digoxin-conjugated ovalbumin in 50 mM sodium bicarbonate buffer (pH 9.6) at 4 °C overnight. After washing with 10 mM phosphate-buffered saline (pH 7.2) (PBS) containing 0.5 ml/1 Tween 20 (T-PBS), the wells were blocked by PBS supplemented with 1 g/1 casein (C-PBS). Fifty pi samples serially... 

Epstein, J. (1948). Studies on hydrolysis of GB I. Effect of pH and temperature on hydrolysis rates II. Observations on hydrolysis of GB in sodium bicarbonate buffered waters, MDR 132, Chemical Warfare Laboratories, Army Chemical Center, Maryland, February 1948, UNCLASSIFIED Report. 

Density, viscosity, and snrface tension of sodium carbonate -1- sodium bicarbonate buffer solutions in the presence of glycerine, glucose, and sucrose from 25 to 40°C ... 

The wells of the microtiter plate were coated with rabbit anti-fluorescein IgG which were 5 pg/mL in 50 mmol/L sodium bicarbonate buffer (pH 9.5). The solution was added 100 pL to each well. The wells were then post-coated by adding 100 pL of a 1% water-soluble gelatin solution containing 0.05% NaN3. The plates were stored at 4°C prior to use. After washing the plate, 100 pL of a PCR product solution were added to each well. The plate was incubated for 1 h at room temperature. After washing, 150 pL of b-Luc/streptavidin complex (1 xl0"9 mol/L), Aq-labeled anti-Dig Fab fragment (495 ng/mL) and HRP-labeled anti-DNP (500-fold dilution) mixed solution was added and allowed to stand for 1 h at room temperature. The microtiter plate was re-washed and Aq, b-Luc and HRP activities were assayed by the simultaneous bio- and chemiluminescent method described above. 

The hydrolysis of glycine amide hydrochloride in sodium carbonate-sodium bicarbonate buffers is promoted by copper(II) and to a lesser extent by eobalt(II) and nickel(II) (148). The presence of 0.02 M copper(II) between pH 7.9 and 9.3 increases the rate of hydrolysis by a factor of about 30. This effect of metal ions on the rate of hydrolysis of glycine amide is surprisingly small when compared with the effect on the rate of hydrolysis... 

Unlabeled proteins are dissolved at 1-10 mg/ml (small proteins 1-3 mg/ml, large proteins or antibodies 1-10 mg/ml) in 0.1 M sodium phosphate buffer (pH 8.0) or 0.1 M sodium carbonate-sodium bicarbonate buffer (pH 8.0) and stored at 4 or -20 °C (see Note 1). Some fluorescent dye-labeled lectins are commercially available from Vector Laboratories, Burlingame, CA, USA or Sigma, St Louis, MO, USA. 

Cy3- or Cy5-labeled proteins are obtained by reacting proteins with Cy3-NHS or Cy5-NHS, respectively. Dissolve the protein (small proteins 0.1-0.3 mg, large proteins or antibodies 0.1-1 mg) in 100 pi of 0.1 M sodium carbonate-sodium bicarbonate buffer (pH 8.0). Do not use buffers containing free amino groups such as Tris or glycine. These buffers will react with Cy3-NHS and Cy5-NHS. 

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