Anti-digoxin antibodies

M2. Mudgett-Hunter, M., Anderson, W., Haber, E., and Margolies, M. N., Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies. Mol. Immunol. 22, 477-488 (1985). 

Intestinal ischemia responds to verapamil and to anti-digoxin antibody (115). 

A monoclonal anti-digoxin antibody (mouse) used for the ELISA was produced and prepared by Sawada et al. [121]. An ELISA plate (96 well) was coated with ca. 100 ng/well digoxin-conjugated ovalbumin in 50 mM sodium bicarbonate buffer (pH 9.6) at 4 °C overnight. After washing with 10 mM phosphate-buffered saline (pH 7.2) (PBS) containing 0.5 ml/1 Tween 20 (T-PBS), the wells were blocked by PBS supplemented with 1 g/1 casein (C-PBS). Fifty pi samples serially... 

Table 17. Cross-reactivity of anti-digoxin antibody in ELISA...

R Stenzel, B Reckmann. Cross-reactivity of anti-digoxin antibodies with digitoxin depends on tracer structure. Clin Chem 38 2228, 1992. 

Table 2. Cross-reactivity of monoclonal anti-digoxin antibody (64. 2B.5) with compounds structurally related to digoxin in ELISA (after Yoshimatsu et al. 1990).

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