Snaps, attaching

Butane cylinders, which are so widely used for domestic service, are now provided with a self-sealing clip-on valve. The pressure regulator, which is normally attached directly onto the cylinder outlet, is fitted by a simple push-on or snap-on action and is provided with a lever, which will open or shut the cylinder valve. 

SNAPs is an acronym for soluble NSF attachment proteins. They were originally discovered as cofactors for NSF that mediate the membrane binding of NSF in in vitro transport assays. Several isoforms of SNAPs exist in mammalian cells. SNAPs are also highly conserved proteins. Crystallographic studies indicated that the proteins form a very stiff and twisted sheet that is formed by a series of antiparallel and tightly packed helices connected by short loops. 

Step 6 The general fusion machinery then assembles on the paired SNARE complex it includes an ATPase (NSF NEM-sensitive factor) and the SNAP (soluble NSF attachment factor) proteins. SNAPs bind to the SNARE (SNAP receptor) complex, enabling NSF to bind. 

Here you use a loaded melting point capillary tube (see Loading the Melting Point Tube ) and attach it directly to the thermometer. The thermometer, unfortunately, has bulges there are some problems, and you may snap the tube while attaching it to the thermometer. 

An early problem was that the blowing process as originally developed produced rounded bases, and so the bottles could not be stacked upright on shelves. Initially, bottles were equipped with separately moulded base cups, usually made from polyolefin and readily attached by a snap-on or glue-on process. The Continental Group then introduced in the USA a bottle with a shaped multilobal bottom that did not require a base cup, and further designs have followed [64],... 

When the bomb equipped with Ml29 fu2e is released from the cluster, the butterfly wings snap open and ride to the top of the cable attached to the arming stem. The... 

Presynaptic nerve terminals may contain as few as a hundred vesicles, which must be recycled rapidly after exocytosis in order to allow for repetitive fir-jHg 558,559,583 gevera proteins are needed for endocyto-sis (step 7 in Fig. 30-20). These include endophilin I,584 the vesicle transport ATPase NSF,574 GTPases,565 and the soluble NSF attachment protein a-SNAP (which is not related to SNAP-25).585... 

Synaptic exocytosis involves three SNARE proteins the R-SNARE synaptobrevin/VAMP (isoforms 1 and 2) on the vesicle, and the Q-SNAREs syntaxin (isoforms 1 and 2) and SNAP-25 on the plasma membrane (Figure 4). Since SNAP-25 has two SNARE-motifs, synaptobrevin, syntaxin, and SNAP-25 together have four SNARE-motifs. Synaptobrevins and SNAP-25 are relatively simple SNARE proteins that are composed of little else besides SNARE motifs and membrane-attachment sequences (a transmembrane region for synaptobrevin, and a cysteine-rich palmitoylated sequence for SNAP-25). Syntaxins, in contrast, are complex proteins. The N-terminal two-thirds of syntaxins include a separate, autonomously folded domain (the so-called Habc-domain), while the C-terminal third is composed of a SNARE motif and transmembrane region just like synaptobrevin. 

Dissociated neuronal cultures provide a versatile system for analysis of mechanisms underlying neurotransmitter release. These cultures can be prepared from fetal or postnatal brain tissue. This preparation has been particularly instrumental in analysis of synapses deficient in key components of the release machinery. For instance, genetic deletion of synaptic SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) proteins such as synaptobrevin-2 and SNAP-25... 

Prepare a trial pool in a snap cap vial consisting of 50 mm3 of each fraction that meets the required specification by HPLC. Label the pool PX/FY-Z, where X is the purification number and Y to Z covers the fraction range in the pool. Dilute the pool 10 times and carry out a HPLC of this pool to confirm that the pool meets the required specification. Attach the HPLC traces to this process instruction. Record the following ... 

The outside walls of the curtain storage rooms have been furred out with two-by-fours covered with plywood and painted. This false wall has a 4" spacing behind to prevent condensation. Box valances are held in place on the walls with L-shaped brackets flat valances are attached by means of snaps or Velcro tape. There are no windows in the storage rooms and the rooms are kept in total darkness. 

Beta-soluble N-ethylmaleimide-sensitive factor attachment protein (Beta SNAP) D Yoo et al. 2001a... 

Synaptosomal proteins are involved in synaptic transmission, which is deranged in AD. The machinery consists of synaptosomal proteins, including soluble N-ethylmaleimide-sensitive factor attachment proteins (SNAPs) present as three isoforms, a, (3 and y, synaptosomal-associated protein 25 (SNAP 25), synapto-tagmin, and vesicular proteins. Proteomics studies showed reduced expression... 

A second, new class of processes is that of membrane and micro-channel emulsification. A to-be-dispersed phase is here pushed through pores of a membrane or through micro-engineered micron-scale channels. At the pore or channel mouth, droplets are formed. These droplets can spontaneously detach from the pore or channel mouth (interfacial tension driven snap-off), due to the distortion of the droplet shape when it is still attached to the mouth. At higher fluxes or with channel mouths not giving a strong shape distortion, droplets are sheared off by a cross-flowing continuous phase. 

Soluble N-ethylmaleimidesensitive factor (NSF) attachment protein (y-SNAP) is another protein found to be oxidatively modified in AD brain, and this protein is important in vesicular transport for neurotransmitter release, hormone secretion, and mitochondrial integrity. Hence, oxidation may lead to an altered neurotransmission system and impaired learning and memory in AD (Masliah et al, 1994 Scheff and Price, 2003 Sultana et al, 2006d). 

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