Single molecule observation

Betzig E and Chichester R J 1993 Single molecules observed by near-field scanning optical microscopy Science 262 1422... 

Figure Cl.5.14. Fluorescence images of tliree different single molecules observed under the imaging conditions of figure Cl.5.13. The observed dipole emission patterns (left column) are indicative of the 3D orientation of each molecule. The right-hand column shows the calculated fit to each observed intensity pattern. Molecules 1, 2 and 3 are found to have polar angles of (0,( ))=(4.5°,-24.6°), (-5.3°,51.6°) and (85.4°,-3.9°), respectively. Reprinted with pennission from Bartko and Dickson [148]. Copyright 1999 American Chemical Society.

Katano, S., Herceg, E., Trenary, M., Kim, Y. and Kawai, M. (2006) Single molecule observations of the adsorption sites of methyl isocyanide on Pt(lll) by low-temperature scanning tunneling microscopy. J. Phys. Chem. E, 110, 20344-9. 

SOURCE Reprinted with permission from Betzig, E., and R J. Chichester. 1993. Single molecules observed by near-field scanning optical microscopy. Science 262 1422-1425. Copyright 1993 American Association for the Advancement of Science. 

Bowen ME, Weninger K, Brunger AT, Chu S. Single molecule observation of liposome-bilayer fusion thermally induced by soluble N-ethyl maleimide sensitive-factor attachment protein receptors (SNAREs). Biophys. J. 2004 87 3569-3584. 

Single-molecule observations are clearly demanding. To observe a single absorbing molecule within trillions of solvent or matrix molecules represents a significant technical problem. In order for this to be possible, two conditions should be fulfilled ... 

The initial experiments discussed above focused on single-molecule observations (i.e., without an injection step), and the matrix was enclosed in a custom electrophoresis cell. A preliminary report [38] using an array of microchannels to sculpt the field [37] indicates that this matrix can be used as an analytical tool. Moreover, pulsed-field separation are possible by mimicking the DNA prism protocol [37,38]. 

Takada T, Fujitsuka M, Majima T. Single-molecule observation of DNA charge transfer. Proc Nat Acad Sci USA. 2007 104 11179-11183. 

Evanescent wave microscopy or total internal reflection microscopy (TIRM) has been employed in the fields of biology and chemistry since the 1970s. The TIRM technique has long been used in cell biology studies and more recently cell-substrate contacts, vesicle fusion, and single-molecule observation. Here, cells on a microscope cover glass are illuminated by an... 

Irreversibility is not meant in a purely thermodynamic sense but more from a kinetic understanding we denote reactions as irreversible when the backreaction is unlikely to occur within the time frame of observation. Those transformations impose several obstacles to their implementation in single-molecule research. Under practical considerations, only reactions in which their onset is triggered by the excitation Ught are susceptible to continuous single-molecule observation by the widely used photon-counting point detectors. All other reactions demand... 

Figure 3.10 Single-molecule observation of the epoxidation reaction of single immobilized BODIPY substrate molecules, (a) TIRFM images taken from the same region at two different time points and two distinct spectral signatures of the substrate (left 570-615 nm) and the product (right 500-525 nm). The product emission was recorded after reaction with 0.2 mM mCPBA in dichloromethane (DCM). (b) Two...

Takei, Y., lizuka, R., Ueno, T., Fimatsu, T. Single-molecule observation of protein folding in symmetric GroEL-(GroES)2 complexes. J. Biol. Chem. 287,41118-41125 (2012)... 

Yoshikawa, Y Hizume, K. Oda, Y Takeyasn, K. Araki, S. Yoshikawa, K. Protective effect of vitamin C against double-strand breaks in reconstitnted chromatin visualized by single-molecule observation. Biophys. J. 2006, 90, 993-999. 

Xie, H. Z. Braha, O. Gu, L. Q. Cheley, S. Bayley, H. Single-molecule observation of the catalytic subunit of cAMP-dependent protein kinase binding to an inhibitor peptide. Chem Biol 2005,12,109-120. 

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