SILAC stable isotope labeling by amino acids

SILAC (stable isotope labeling by amino acids in cell culture) is an in vivo labeling technique. 

SILAC. Stable Isotope Labeling by Amino Acids in Cell Culture, http //www.silac. org/ (accessed May 17,2015). 

Later, Ong et al. introduced a method termed SILAC (stable isotope labeling by amino acids in cell). In SILAC, two cell-culture populations are grown under identical conditions, except that one is supplied with the labeled amino acids (e.g. arginine with six atoms) and the other is with the non-labeled. After five or six doublings, two kinds of amino acids have fully incorporated into proteins. Every peptide pair is separated by the mass shift by the labeled amino acid. This approach cannot be applied to tissues or body fluids, and is... 

The approach recruited to chemical proteomics in Reference [17] is called SILAC (stable isotope labeling with amino acids in cell culture) and is important in comparative proteomics (Figure 1). SILAC works well with cultured mammalian cells, but prokaryotes defeat it by metabolizing the label (usually supplied in lysine and arginine) into other amino acids. For applications beyond cultured eukaryotic cells, the reductive methylation route to differential labeling [18] is among the alternatives [15]-... 

Ong, S.-E., Blagoev, B., Kratchmarova, I., Kristensen, D.B., Steen, H., Pandey, A., and Mann, M. (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 1, 376-386. 

Demirev et al. 2013). A partially labeled ( locally labeled ) medium contains a particular component that is labeled, for example, and/or N-labeled-specific amino acids. The second approach has been introduced more than 10 years ago as a major step in MS-based quantitative proteomics approaches. Stable isotope labeling by amino acids in cell cultures (SILAC Mann 2006) is the such a major protocol, and it has been used extensively for identification and quantitative evaluation of the expression levels of individual cellular proteins imder various conditions. 

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