Stable isotope labeling by amino acids

Ong, S.-E., Blagoev, B., Kratchmarova, I., Kristensen, D.B., Steen, H., Pandey, A., and Mann, M. (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 1, 376-386. 

Quantification of Proteins with Stable-Isotope Labeling by Amino Acids in Cell Culture 469... 

SILAC (stable isotope labeling by amino acids in cell culture) is an in vivo labeling technique. 

N. Ibarrola, D. E. Klume, M. Gronborg, A. Iwahori, and A. Pandey, A proteomic approach for quantitation of phosphorylation using, stable isotope labeling by amino acids in cell culture. Anal. Chem. 75, 6043-6049 (2003). 

Warscheid, B. (ed.) (2014) Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Methods and Protocols (Methods in Molecular Biology). Humana Press, New York. 

Demirev et al. 2013). A partially labeled ( locally labeled ) medium contains a particular component that is labeled, for example, and/or N-labeled-specific amino acids. The second approach has been introduced more than 10 years ago as a major step in MS-based quantitative proteomics approaches. Stable isotope labeling by amino acids in cell cultures (SILAC Mann 2006) is the such a major protocol, and it has been used extensively for identification and quantitative evaluation of the expression levels of individual cellular proteins imder various conditions. 

Later, Ong et al. introduced a method termed SILAC (stable isotope labeling by amino acids in cell). In SILAC, two cell-culture populations are grown under identical conditions, except that one is supplied with the labeled amino acids (e.g. arginine with six atoms) and the other is with the non-labeled. After five or six doublings, two kinds of amino acids have fully incorporated into proteins. Every peptide pair is separated by the mass shift by the labeled amino acid. This approach cannot be applied to tissues or body fluids, and is... 

---