Signal transduction enzymes

Keywords Bioorganic chemistry, Cellular signal transduction, Enzyme-Inhibition, Ras-farne-syltransferase, Tumor-therapy... 

EA Dennis. The growing phospholipase A2 superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2, 1997. 

The organic extract, freeze-dried extract and freeze-dried residue were tested for brine shrimp lethality, signal transduction enzyme inhibition, and antimicrobial activity. Microbes with promising activity were tagged for further study using the physicochemical conditions that produced maximum biological activity. 

Table 2. Signal Transduction Enzyme Inhibition Profiles for Microbial Extracsts...

Signal transduction enzyme inhibition assays guided the isolation of two novel hybrid polyketide-terpenoid metabolites from a Penicillium sp. growing in the deepest waters (>750 ft) of Berkeley Pit Lake [9], Their structures were deduced by spectroscopic analysis and confirmed by single crystal x-ray analysis on berkeleydione (13). Both compounds inhibited signal transduction enzymes caspase-1 and matrix metalloproteinase-3. Berkeleydione (13) was also active against non-small cell lung cancer in NCI s 60 cell line anti-tumor screen. 

The crude organic extracts of a Penicillium sp. isolated from a depth of 885 ft. were active against Staphylococcus aureus and in the brine shrimp lethality screen. These extracts were further tested using enzyme inhibition assays for two different signal transduction enzymes - matrix metalloproteinase-3 (MMP-3) and caspase-1 (Casp-1). Compounds 13 and 14 were isolated from the chloroform extracts of the broth filtrate of a Penicillium sp. found growing in Berkeley Pit Lake. Berkeleydione (13, 5.5 mg/L) was isolated as a crystalline... 

Arkin, M.R. and Whitty, A. (2009) The road less traveled modulating signal transduction enzymes by inhibiting... 

---