Fluorescent signal

To date, effective, positive-signaling fluorescent chemosensors have been... 

Chapter 9 describes how single photon and two-photon fluorescence may be exploited in biology and more specifically in molecular signalling. Fluorescence... 

Read the plate immediately or up to 2 h after the end of the incubation on a 8200 cellular detection system (Appfted Biosystems) or on an equivalent analyzer. Positive signals (fluorescence) indicate that mAb successfully bound to GAG displayed chemokines. 

Triton X-100 is injected to lyse cells and obtain the maximal internal signal, fluorescent trace is recorded for 10 s. 

The same principles of total internal reflection can also be used to excite fluorescent chromophores attached to the polymer chain (9). The emitted fluorescence can be used to estimate the surface excess. In addition to subtracting the background signal, fluorescence quenching in the adsorbed layers may also have to be considered while estimating the adsorbed amount. The disadvantage of this technique is that the polymer chains have to be labeled with fluorescence molecules and that might influence the adsorption process. 

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