Signal-amplified fluorescent probing

Signal-amplified Fluorescent Probing Techniques Metallic nanoparticle enhancedfluorescence... 

There are two primary types of qPCR the first involves the use of fluorescence reagents that are intercalated into the double-strand structure of the amplified PCR product (i.e., the intercalation method), and the other involves the use of a probe molecule that yields dual fluorescent signals (i.e., the probe method) [37]. The former is less costly than the probe method because an expensive fluorescence probe is not required. However, use of the intercalation method can also result in nonspecific amplification by intercalation of the fluorescence reagents into an undesired PCR product. Therefore, qPCR assays using the intercalation method should include a dissociation curve to examine nonspecific amplification. If double... 

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