Sheep red cells

Usually sheep red cells (SRBC) are used human type O cells are equally suitable. Pooled SRBC are better than cells from single animals they are available commercially in the form of whole pooled blood in modified citrate solution containing about 25% red cells. Such blood samples are stable at 4° for at least 1 month they should be discarded at the first sign of spontaneous lysis or lysis during washing. Coated cells, prepared by either of the two procedures to be described, are stable for 1 week at 4°. 

Para th ion Male mice, oral, 16 mg/kg single dose, 2 days after immuni/ation with sheep red cells Suppression of primary IgM plaque-forming cells Ca.salc ei ai (1983)... 

Do.se-related decrease in splenic plaque-forming cells after sensitizing with sheep red cells after inhalation... 

C3, C9 and factor B, without alteration of C4 or C5 levels. Additionally, our own studies in Guatemala (Keusch et al., 1980a) show a significant and reversible abnormality in hemolytic complement activated by the classical or alternative pathways, determined by a quantitative kinetic hemolytic assay using antibody sensitized sheep red cells to assay the classical pathway and washed rabbit red cells for the alternative pathway. The abnormalities reversed to normal within 4 weeks of optimal nutritional rehabilitation. 

Burro red cells (BRC) were added to spleen cell suspensions from normal mice at time 0. Sheep red cells (SRC) and a hot pulse (10 (xC of tritiated thymidine in 0.16 (xg thymidine per 1 ml culture) were added 24 hours later. Thus, in the same culture, the response to BRC was pulsed at 24-48 hours after addition of BRC, while the response to SRC was pulsed at 0-24 hours after addition of SRC. It can be seen in Table 8 that the response to BRC is completely inhibited, while the response to SRC is normal. 

The second prediction was tested by Radovich and Talmage (1967). Lethally irradiated mice received transfers of 10 or 5 X 10 normal spleen cells on the same day as transfer, horse red cells were also injected and sheep red cells were given four days later. The results of the experiment showed, that contrary to what would be expected if the cells were uncommitted, the competition was more pronounced at the higher cell concentration. 

Quintans, J., Lefkovits, I. Precursor cells specific to sheep red cells in nude mice. Estimation of frequency in the microculture system. Europ. J. Immunol. 3, 392-397... 

Kramer, R., Schlatter, C., and Zahler, P., 1972, Preferential binding of sphingomyelin by membrane proteins of the sheep red cell, Biochim. Biophys. Acta 282 146. 

Bentwich, Z., Douglas, S. D., Skutelsky, E., and Kunkel, H. G., 1973, Sheep red cell binding to human lymphocytes treated with neuraminidase enhancement of T cell binding and identification of a subpopulation of B Cells, J. Exp. Med. 137 1532-1537. 

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