SH-SY5Y neuroblastoma

SH-SY5Y neuroblastoma, cisplatin-sensitive A2780 and cispla-tin-resistant A2780cis human ovarian cancer cells was observed, but upon irradiation 7 strongly reduced the viability of the cancer cells (Fig. 8). In the A2780 cell line, the complex was 80x more toxic than cisplatin under identical conditions, and ca. 15 x more effective against the cisplatin-resistant A2780cis cell line (33). The trans diazido-Pt(IV) complex therefore has remarkable cytotoxic properties. 

Liu, X. G., Evans, A. E., Beodeue, G. M. (2002). Expression of the neurotrophin receptor TrkA down-regulates expression and function of angiogenic stimulators in SH-SY5Y neuroblastoma cells. Cancer Res. 62, 1802-1808. 

McGinnis, K.M., Gnegy, M.E., Falk, N., Nath, R., and Wang, K.K.W. 2003. Cytochrome c translocation does not lead to cas-pase activation in maitotoxin-treated SH-SY5Y neuroblastoma cells. Neurochemistry International 42, 517-523. 

Wang, K.K.W., Nath, R., Raser, K.J., and Hajimohammadreza, I. 1996. Maitotoxin induces calpain activation in SH-SY5Y neuroblastoma cells and cerebrocortical cultures. Archives of Biochemistry and Biophysics 331, 208-214. 

Notes Human SH-SY5Y neuroblastoma cells were Incubated for 1 h with different concentrations of each organophosphate (OP). Cytotoxicity was measured by the neutral red assay. Results represent the IC50 values for inhibition of AChE and NTE activities and for cytotoxicity. DBVP = 0,0-dibutyl 0-(2,2-dlchlorovlnyl) phosphate DOVP = 0,0-dloctyl 0-(2,2-dlchlorovlnyl) phosphate. A high ratio of NTE IC50/AChE IC50 Indicates that the OP is likely to cause acute rather than delayed neurotoxic effects in vivo. 

McLean WG, Holme AD, Janneh O, et al. The effect of benomyl on neurite outgrowth in mouse NB2A and human SH-SY5Y neuroblastoma cells in vitro. Neurotoxicology. 1998,19 629-632. 

Combretafurazans (1,2,5-oxadiazole) are more potent in vitro cytotoxic compounds compared to combretastatins in SH-SY5Y neuroblastoma cells (yet maintaining similar structure-activity relationship and pharmacodynamic profiles). In particular the fiirazan derived from Combretastatin A-4 and 3 -deoxy-3 amino Combretastatin A-4 exhibited IC50 values about 4 times lower with respect to Combretastatin A-4. 

Selected Changes in Gene Expression of SH-SY5Y Neuroblastoma Cells Stimulated With Norepinephrine... 

Jia Z, Misra HP. Exposure to mixtures of endosulfan and zineb induces apop-totic and necrotic cell death in SH-SY5Y neuroblastoma cells in vitro. J Appl Toxicol2007 27(5) 434 46. 

Azmi NH, Ismail N, Imam MU, Ismail M (2013) Ethyl acetate extract of germinated brown rice attenuates hydrogen peroxide-induced oxidative stress in hitman SH-SY5Y neuroblastoma cells Role of anti-apoptotic, pro-survival and antioxidant genes. BMC Complement Altern Med 13 177... 

Cho DH, Lee EJ, Kwon KJ, Shin CY, Song KH, Park JH et al (2013) Troglitazone, a thiazolidinedione, decreases tau phosphorylation through the inhibition of cyclin-dependent kinase 5 activity in SH-SY5Y neuroblastoma cells and primary neurons. J Neurochem 126 685-695... 

Arias, E. et al., Unequal neuroprotection afforded by the acetylcholinesterase inhibitors galantamine, donepezil, and rivastigmine in SH-SY5Y neuroblastoma cells role of nicotinic receptors, J. Pharmacol. Exp. Ther., 315, 1346, 2005. 

Grudt TJ, Usowicz MM, Henderson G. 1996. Ca2+ entry following store depletion in SH-SY5Y neuroblastoma cells. Mol Brain Res 36 93-100. 

NMR of neuroblastoma cells treated with COX inhibitors showed accumulation of polyunsaturated fatty acids and depletion of choline compounds. The effects of the presence or absence of serum in the growth medium of human SH-SY5Y neuroblastoma cells has been investigated with NMR. Chronic serum deprivation resulted in a decrease of several intracellular metabolites, particularly choline. A drop in lipid methylene protons was observed shortly after withdrawal of serum. However, removal of lipoproteins from the serum resulted in a pronounced increase in intracellular lipids. A similar effect was seen after treatment of cells with lovastatin. ... 

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