Serum isocitrate dehydrogenase

K8. Kerppola, W., Nikkila, E. A., and Pitkanen, E., Serum TPN linked enzymes glucose-6-phosphate dehydrogenase, isocitric dehydrogenase and glutathione reductase activities in health and various disease states. Acta Med. Scand. 164, 357-305 (1959). 

Fujita T, Kawakami Y, Kohda S> Takata S> Sunahara Y, Arisue K. Assay of magnesium in serum and urine with use of only one enzyme, isocitrate dehydrogenase CNADP+). Clin Chem 1995 41 1302-5. 

The isocitrate dehydrogenase activity in human serum has been determined by kinetic assay at 37°C, with isocitrate and NADP as substrates. The rate of formation of NADPH was amperometrically monitored and the selectivity was improved by covering the active surface of the electrode with a poly(o-phe-nylenediamine) film. The electroimmobilization of the enzyme monoamine oxidase into a polypyrrole film around a platinum electrode has been used to determine the antidepressant drug fluoxetine in pharmaceutical preparations. 

Nicotinamide adenine dinucleotide phosphate, which occurs in oxidized (NADP+) and reduced (NADPH) forms with markedly different absorption spectra and an absorptivity similar to NAD can also be used for enzymatic analysis. Thus, Mg in serum can be measured by the oxidation reaction of isocitrate with NADP+, catalyzed by isocitrate dehydrogenase (IDH) (eqn [HI]) ... 

Serum liver enzymes are elevated, including aspartate transaminase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (AP), ornithine carbamyl transferase, and isocitric dehydrogenase. 

Hemoglobin A2 (a5) 2. Isocitric dehydrogenase 2. Peptidase D 2. Serum amylase... 

W18. Wolfson, S. K., Jr., and Williams-Ashman, H. G., Isocitric and 6-phosphogluconic dehydrogenases in human blood serum. Proc. Soc. Exptl. Biol. Med. 96, 231-234 (1957). 

Hemolysis is defined as the disruption of the red cell membrane and results in the release of hemoglobin. Serum shows visual evidence of hemolysis when the hemoglobin concentration exceeds 200 ing/L. Slight hemolysis has little effect on most test values. Severe hemolysis causes a slight dilutional effect on those constituents present at a lower concentration in the erythrocytes than in plasma. However, a notable effect may be observed on those constituents that are present at a higher concentration in erythrocytes other than in plasma. Thus plasma activities or concentrations of aldolase, total acid phosphatase, lactate dehydrogenase, isocitrate dehydro-... 

---