Serum amyloid P component

Heegard, N. H. H. and Robey, R A., Use of capillary zone electrophoresis to evaluate the binding of anionic carbohydrates to synthetic peptides derived from human serum amyloid P component, Anal. Chem., 64, 2479, 1992. 

CAIA, collagen Ab-induced arthritis CIA, collagen-induced arthritis SAP, serum amyloid P-component. 

Pepys, M.B., Herbert, J., Hutchinson, W.L., et al. (2002) Targeted pharmacological depletion of serum amyloid P component for treatment of human amyloidosis. Nature, 417, 254-259. 

NHH Heegaard, HD Mortensen, P Roepstorff. Demonstration of a heparinbinding site in serum amyloid P component using affinity capillary electrophoresis as an adjunct technique. J Chromatogr A 717 83—90, 1995. 

NHH Heegaard, PMH Heegaard, P Roepstorff, FA Robey. Ligand-binding sites in human serum amyloid P component. Eur J Biochem 239 850—856, 1996. 

Hawkins. P. el ah F.valuation of Systemic Amyloidosis by Scinngraphy with 1231-Labeled Serum Amyloid P Component. iV. /.lie. / Met .. SDH (August 23. 199tii. 

Heegaard et al. [8] seem to be the first who used this approach to separate free synthetic peptide derived from the heparin-binding region of human serum amyloid P component from its complex with anionic carbohydrates. Quantification of the peptide in a series of affinity mixtures was accomplished by a comparison of the relevant peak areas with the calibration curve. Using UV detection, the authors obtained a dissociation constant of about 10 s M, which is in the range of the sensitivity such detection can provide. 

Llovat, L.B., Persey, M.R., Madhoo, S., Hawkins, P.N. The liver in systemic arrtyloidosis insights from 1231 serum amyloid P component scintigraphy in 484 patients. Gut 1998 42 727 -734... 

De Haas CJ. New insights into the role of serum amyloid P component, a novel lipopolysaccharide-binding protein. FEMS Immunol Med Microbiol 1999 26 197-202. 

Hawkins PN. Serum amyloid P component scintigraphy for diagnosis and monitoring amyloidosis. Curr Opin Nephrol Hypertens 2002 11 649-55. 

M.B. (1994) Concentration of serum amyloid P component in the CSF as a possible marker of cerebral amyloid deposits in Alzheimer s disease, Biochem Biophys Res Commun 201, 722-726. 

Hawkins, P.N., Tyrell, P., Jones, T., et al. (1991) Metabolic and scintigraphic studies with radiolabeled serum amyloid P component in amyloidosis applications to cerebral deposits and Alzheimer disease with positron emission tomography. Bull Clin Neu-rosci, 178-190. 

Pepys, M. B. Serum C-reactive protein, serum amyloid P-componant and semm amyloid A protein in autoimmune disease. Clin. Immunol. Allergy 77-101 (1981). 

Pepys, M. B., M. Baltz, K. Gomer, A. J. S. Davies, and M. Doenhoff. 1979. Serum amyloid P-component is an acute-phase reactant in the mouse. Nature 278 259-261. 

Steel, D., and Whitehead, A.S., 1994, The major acute phase reactants C-reactive protein, serum amyloid P component and serum amyloid A protein. Immunology Today 15 81-88. 

Figure 9. X-ray structure of human serum amyloid P component with modelled oligosaccharide chains.

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