Selection Sequence

Probably the most common method used for sequence selection for simple distillation columns is heuristic. Many heuristics have been proposed, but they can be summarized by the following four ... 

Titanium tetrakis(diethylamide) selectively adds to aldehydes in the presence of ketones and to the least hindered ketone in compounds containing more than one ketone. The protection is in situ, which thus avoids the usual protection-deprotec-tion sequence. Selective aldol and Grignard additions are readily performed employing this protection methodology. ... 

Dahiyat, B.I., Mayo, S.L. De novo protein design fully automated sequence selection. Science 278 82-87,... 

Nielsen P.E., Egholm M., Berg R.H., Buchardt O. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 1991, 254 1497-1500. 

In an effort to improve the water solubility of camptothecin, Rahier et al. [50] synthesized four 20-O-phosphate derivatives (VI). These compounds are freely water-soluble, stable to physiological pH, and stabilize the human topo I-DNA covalent binary complex with the same sequence-selectivity as camptothecin itself All four compounds inhibited the growth... 

This assay system developed by Chaires [136] is a new, powerful and effective tool based on the fundamental thermodynamic principle of equilibrium dialysis for the discovery of ligands that bind to nucleic acids with structural and sequence selectivity. Here, identical concentrations of various nucleic acid samples are dialysed in dispodialysers against a common ligand solution. At equilibrium, the contents of the ligand bound to each nucleic acid are determined and this is correlated directly to the ligand s specificity to a particular sequence. 

Earlier studies using thermal denaturation analysis and spectrophotomet-ric titration with TxA T and CxC-C" containing DNA triplexes showed that coralyne binds strongly to these triplexes by intercalation and does not exhibit a significant sequence-selectivity [222]. In a later study by Morau Allen et al. [217], employing DNase footprinting, thermal denaturation analysis, UV-visible spectrophotometric titrations, circular dichroism and NMR spectroscopy, showed that coralyne is fully intercalated into TxA T triplex DNA whereas in C GxC triplex, it is partially intercalated due to electrostatic repulsion between the cationic alkaloid and the protonated cytosine [217]. Kepler et al. [223] demonstrated that coralyne intercalated to parallel triplex DNA but did not intercalate to antiparallel triplex DNA. Recently Hud and coworkers [219,224] demonstrated that duplex poly(dA) poly(dT) is trans-... 

Below, the procedure for the determination of dominant campaigns in a version that was proposed by Lazaro et al. (1989) is outlined. Their methodology includes enumeration of feasible production sequences, selection of dominant production lines, task sequencing, and search for an optimum with constraints. All possible production variants are generated by an enumeration procedure that takes into account the possibility of available equipment working in parallel, initial and final task overlapping, and instability of intermediate products. Non-feasible sequences are eliminated so that only favourable candidates are subjected to full evaluation. Dominant production lines are selected by maximizing the criterion ... 

Nakatani, K. Matsuno, T. Adachi, K. Hagihara, S. Saito, I. Selective intercalation of charge neutral intercalators into GG and CG steps implication of HOMO-LUMO interaction for sequence-selective drug intercalation into DNA. J. Am. Chem. Soc. 2001, 123, 5695-5702. 

FIGURE 3.5 Alignment of the TM2 amino acid sequences. The nomenclature of the rings is based on the a7 sequence. Selectivity indicates the charge of the permeant ions. Mutl and Mut2 are site-directed mutants (indicated by the asterisks) of the a7 subunit (see text). 

Nature provides examples of sequence-selective minor groove binding, such as antibiotics including netropsin, distamycin, anthelvencin, and kikumycin. 

The sequence selectivity of demethylation within histones has been established for many of the demethylases (Figure 3) [5]. Demethylase... 

Acquisition of wide static or MAS spectra of the CT is typically made using a two-pulse echo sequence nil t n (the Hahn echo [55,106]). By setting the strength of the rf-field at mrf = Aco/(I+ 1/2), where Aco is the CT linewidth, these sequences selectively irradiate the CT, while minimizing the contributions from the STs [107, 108]. In such case, the opjj frequencies are given by (29) ... 

Murray V, Whittaker J, McFadyen WD (1998) DNA sequence selectivity of cisplatin analogues in intact human cells. Chem Biol Interact 110(l-2) 27-37... 

Amplifies a DNA sequence selected by primers complementary to 3 borders... 

Still, W. C. Discovery of sequence-selective peptide binding by synthetic receptors using encoded combinatorial libraries. Acc. Chem. Res. 1996,29,155-163. 

Tse, W. Boger, D. Sequence-selective DNA recognition Natural products and nature s lessons. Chem. Biol. 2004,11,1607-1617. 

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