Sendai virus binding

High-affinity binding of Sendai virus to G1 gangliosides with a terminal disialosyl group... 

Figure 7. Specific binding of Sendai virus to GQlb ganglioside and its inhibition by antiserum as demonstrated with (a) the VCS method and (b) hemadsorption (see text). A virus in fourfold serial dilutions in buffer B in lower-titer antiserum, 1% C in the same serum, 10% D in higher titer antiserum, 1% this serum, 10%.

It is conceivable that the natural receptor binding structure for Sendai virus has only one terminal NeuAc residue, but it is also possible that some of the oligosaccharide moieties received a disialosyl linkage during the incubation with the specific sialyltransferase. 

Interferon AMa-n3. Interferon alfa-n3 is a glycoprotein produced from cultures of human leucocytes treated with Sendai virus. It is purifled initially by chromatography using a mou.se monoclonal antibody that binds to multiple species of human interferon. Subsequent purification involves incubation at 4°C and pH 2 to kill viruses and gel nitration chromatography. It then has a specific activity of about 2 x 108 lU. The drug is supplied in l-mL vials containing I mL of phosphate buffered saline solution, phenol as a preservative. and I mg of human serum albumin as a stabilizer. This solution should be kept at 2 to 8°C. Therapeutic indications and side effects of interferon alfa-n3 are. similar to those described for interferons alfa-2a and alfa-2b. 

In addition, RNA synthesis is initiated at the 3 end of the genome RNA with the synthesis of a small 48 nucleotide "leader RNA." Determination of the complete nucleotide sequence of the leader RNA demonstrated that unlike any VSV mRNAs it contains 50% AMP and a ppA at its 5 end. ° Two serotypes of VSV and the paramyxovirus, Newcastle disease virus, synthesize leader RNAs of identical size and similar base composition. The VSV leader RNAs share 80% base sequence homology which indicates nearly identical binding and initiation sites for the virion polymerases. The question remains whether the five mRNA species arise by a processing mechanism after synthesis of the leader RNA or a stop-start mechanism in which the RNA polymerase reinitiates at each junction between mRNAs. Similar sequential synthesis of mRNA has also been observed with the paramyxovirus, Sendai virus. 

The ability of the virus to agglutinate erythrocytes has been first reported in 1941. It took more than a decade before it was shown that influenza virus binds to erythrocytes and other cells via 7 -acetylneuraminic acid residues present on the cell surface and that this binding is a prerequisite for initiation of infection [24,25]. Other viruses, such as Sendai, Newcastle disease, polyoma and rotavirus also exhibit an affinity for sialie... 

Interferon and synthetic fragments Sendai virus membrane protein Interleukin I fragment Heparin-binding growth factors fi-Cisein tryptic peptides Bacteriophage T4 gene 43 protein tryptic peptides V-C4 RBC membrane protmns H-C4... 

Changes in the fluidity of erythrocyte membranes on binding influenza or Sendai virus have been ascribed to the interaction between the viral haem-agglutinin and a glycoprotein on the surface of the erythrocyte. ... 

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