Semi-batch cultivation

A marine microalga utilization for a paper Semi-batch cultivation of Tetraselmis sp. Tt-1 by a tubular bioreactor and the partial substitution of whole kenaf pulp for a paper... 

A semi-batch cultivation of a Prasinophyceae, Tetraselmis sp.Tt-1, was examined using a SOL scale tubular bioreactor under lamps with a view to estimating its performance. The mean productivity of more than 20g/mVday could be obtained by fixing the flow rate of the culture medium at 0.5m/s, which was the maximum in this apparatus, by drawing a partial medium once in two days, and by adjusting an initial cell concentration to 0.5-1.0g/L. A long-term and stable cultivation for more than two months could be carried out by this system. 

A microalga used in this study was Tetraselmis sp.strain Tt-1 [1] which was isolated from seawater ladled at the Sagami Bay in Japan. The f/2 medium [5, 6] was prepared as described previously [7], and was used with a slight modification concentrations of nitrate and phosphate were enriched 4 times and 8 times, respectively. The semi-batch cultivation of this strain was carried out using a tubular... 

Bioreactor operation is either batch-wise or continuous. Batch-wise operation or batch cultures include aU the nutrients required for the growth of cells prior to cultivation of the oi anisms. After inoculation, cell growth commences and ceases once the organisms have exhausted all the available nutrients in the culture medium. A modification of this type of operation is a fed-batch or semi-batch operation in which the reactants are continuously fed into the bioreactor, and the reaction is allowed to go to completion, after which the products are recovered. Continuously operated hioreactors, use continuous culture systems that continuously feed culture medium into... 

The bioreactor operation mode is normally defined at the outset of process configuration. Insect cells have been cultured in almost all known cultivation modes batch [10], repeated-batch [70], perfusion [71-74], fed-batch [75, 76], semi-continuous [77,78] and continuous [79]. In spite of this multitude of different strategies, the batch or, eventually, fed-batch mode is normally preferred due to the lytic infection cycle of the baculovirus. 

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