Selection, sole source

The ability to use azo dyes as sole energy and carbon source by bacteria to be able to reduce the azo bond aerobically by a cometabolic way has been reported [2,4]. A mixture of four structurally different dyes (Acid Red 88, Reactive Black 5, Direct Red 81, and Disperse Orange 3) was used as sole source of carbon and nitrogen to select six strains of bacteria tested for the ability to decolorize the dyes individually or in mixtures a S. putrefaciens strain was identified as the most efficient [45]. 

Platform A will be the sole source of gas supply to the onshore plants for some years and therefore considerable emphasis was placed on selecting a conservative design, minimising corrosion by the use of corrosion inhibitors ond alloy materials ond reducing maintenance downtime by providing 100% standby for items which by their nature will require frequent maintenance or cleoning. 

Sole source both equipment purchases and subcontractor selection. 

Specific Microbial Transformations on Defined Media. Volatiles may be produced from precursors which represent the sole source of carbon. The microorganisms selected to accomplish the specific biotransformation may not grow on such a substrate. A sufficient mass of the cells may have to be obtained and then the precursor introduced into the culture in small quantities. In this way the microorganism is "forced" to carry out the "targeted"... 

In a selection strategy based on the substrate 1-phenyl-n-propylamine (PPA) as the sole source of nitrogen in a chemostat, a recombinant Pseudomonas putida strain carrying the R-transaminase gene, a single amino acid change, Y112F, presumably at or near the active site, improved enantioselectivity of the reaction of racemic... 

Table 1 Selection of axenic bacterial cultures with the capacity to grow on MTBE and/or TBA as sole source of carbon and energy... 

The catabolic pathway in microorganisms does not stop at 4. Selective culture techniques were used by Snell and coworkers to identify 10 different bacterial strains and one yeast strain (Table 1), which were able to grow on one or more forms of vitamin Bg as the sole source of carbon and nitrogen. The catabolic intermediates have been isolated and characterized from the growth medium,and two catabolic pathways have been proposed as shown in Figure 1. Both these pathways are inducible and the enzymes catalyzing these steps have also been characterized. 

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