Scale biotechnology-derived products

This chapter covers the general principles involved in the scale-up of biotechnology-derived products. Sections 1 and 11 focus on technologies currently used in the manufacture of commercial products. Sections III and IV include a practical approach to process design and scale-up strategies used to translate process development to large-scale production. 

Due to the complexity of biological systems and the physical and biochemical characteristics of the protein products, the design and scale-up of biological processes can be challenging. Batch sizes for the production of biotechnology-derived products can reach 10,000 L [1] to 12,500 L [2,3] and above. Although these 95... 

C4 dicarboxyUc acids are already identified in the US Department of Energy s list (2004) of potential large-scale biomass-derived chemicals (Werpy and Petersen 2004). In the past decade, succinic acid has established itself as a foreruimer in biorefinery platform chemicals. Compared with traditional synthetic methods by fossil fuels, the production of succinic acid from natural derived biomass would alleviate our dependence on oil supply for the production of these platform chemicals in future. Recognizing the importance of the biotechnological production of succinic acid, herein we intend to review microorganism producers and... 

Hydroxypropanoic acid (3HPA) is under development as a future platform chemical and monomer derived from biomass. It is, at the present time, not produced on an industrial scale, either chemically or biotechnologically. 3HPA could be a key compound for the production of biomass-derived C3 intermediates, such as acrylic acid, acrylic amide and malonic acid (see Fig. 8.11). Hydrogenation of 3HPA would provide a competing procedure for the production of 1,3PD (see Section 8.2.4) that could be more economical than the DuPont and Shell processes [65]. 

Petricciani, J.C. (1985). Regulatory considerations for products derived from the new biotechnology. In Large-scale Mammalian Cell Culture. J.Feder and W.R.Tolbert, eds. (New York Academic Press), pp. 79 86. 

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