Saccharomyces cerevisiae expression vector

Yeast expression vectors have been among those most commonly used since the beginning of gene technology. Vectors based on baker s yeast, Saccharomyces cerevisiae, have been especially popular for robust expression of many types of recombinant proteins [90]. For instance, the first commercially available recombinant vaccine, the hepatitis B surface antigen vaccine, was produced from an S. cerevisiae vector [91]. Many other recombinant proteins have also been efficiently expressed in yeast including al-Antitrypsin [92], insulin [93], Epstein-Barr virus envelope protein [94], superoxide dismutase [95] and interferon-a [90]. 

GlucaGen (glucagon) [expressed in a Saccharomyces cerevisiae vector-recombinant hormone]... 

Miller, C.A., Martinat, M.A. and Hyman L.E. (1998) Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids expression vectors with bidirectional promoters for use in Saccharomyces cerevisiae. Nucleic Acids Research, 26, 3577-3583. 

In the next contribution we learn about hands-on experience and recent improvements with different production systems for biopharmaceuticals at Bayer Health-Care. As previously also published in Nature by Heiner Apeler, Head of Expression, an E. coli host/vector system was originally developed for the efficient production of an interleukin-4 variant, but afterwards it was optimized for the expression of other proteins and even Fab fragments. Process development and optimization of the yeast secretory Saccharomyces cerevisiae for expression of a protease inhibitor will also be presented. The focus, however, is on the use of a recently developed mammalian HKBll (hybrid clone of human kidney and B cells) expression system for recombinant human glycoprotein biopharmaceuticals. HKBll is a favorable cell host for the production of human proteins, because it dehvers biopharmaceuticals that are structurally identical to the natural product. The host/vector system supports the production of gram quantities of proteins in a large-scale transient transfection format as well as the development of stable cell fines. These systems together... 

A.E., van der Aar, P.C., van Heerikhuizen, H. et al. (1989) High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae a new vector for high-level expression. Gene, 79, 199-206. 

Partow S, Siewers V, Bjpm S, Nielsen J, Maury J (2010) Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 27(11) 955-964. doi 10.1002/yea. 1806... 

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