Fixed-Angle Rotors

Most laboratories have a standard low-speed centrifuge used for routine sedimentation of relatively heavy particles. The common centrifuge has a maximum speed in the range of 4000 to 5000 rpm, with RCF values up to 3000 X g. These instruments usually operate at room temperature with no means of temperature control of the samples. Two types of rotors, fixed angle and swinging bucket, may be used in the instrument. Centrifuge tubes or bottles that contain 12 or 50 mL of sample are commonly used. Low-speed... 

Benchtop centrifuge lEC Model HN-SII 6 x 50-mL fixed-angle solid rotor, 15-mL adapter sleeves Beckman pH meter... 

Acceleration/deceleration fast/fast. Many investigators overlook the affect that improper acceleration or deceleration can have on disrupting the separation, especially when re-orientation of the gradient occurs in fixed angle or vertical tube rotors. SpinPro addresses many special cases. 

Fig. 5.2. rpm-g graph for fixed-angle (type 45 Ti) and swinging-bucket (SW 28) Beckman Coulter ultracentrifuge rotor... 

Free subcellular particles or virus from Percoll by centrifuging the respective fraction of cell fractionation in a fixed-angle rotor at 100 000 X g for 90 min. Percoll forms a solid pellet whidi contains practically no material of interest. 

Rotors for a high-speed centrifuge. A Fixed angle B swinging bucket C vertical. Courtesy of Beckman Instruments, Inc. 

Operation of a fixed angle rotor. A Loading of sample. 

Operation of a fixed angle rotor A Loading of sample B Sample at start of centrifugation C Bands form as molecules sediment D Rotor at rest showing separation of two components... 

Figure 10.5 Pore water extraction by (A) drainage centrifugation using a swing-out rotor and (B) immiscible liquid displacement using a fixed-angle rotor (after Kinniburgh and Miles, 1983).

Beckman Instruments. (1988). Instructions for Using the JA-10 Fixed Angle Rotor. Beckman J-21 Series, J—6, andJ-6B. Spinco Division of Beckman Instruments, INC., Palo Alto, CA. 

Add sterile water to the neutralized ssDNA solution in the Centricon-100 tube to a total volume of 2.0 ml. Spin for 25 min in a fixed-angle rotor at the highest speed recommended for the Centricon tubes. Add... 

Thermostatized centrifuge with fixes angle rotor (angle of inclination 33°) (e.g. Hettich Rotixa RP 001260 Tuttlingen Germany)... 

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