Riboses, identification

Adenosine-0 OH (34) 0-P=0 Insect eggs. 400 MHz 31p 61.0 CO3OO C-22 678.5 (apoc=4 Hz) - shifted downfield 4.6 ppm from ecdysone l C aided ribose identification. iH aided adenine identification. Phosphate conj. at C-22 also reported. 

Miwa M, Saikawa N, Yamaizumi Z et al. Strucmre of polyfadenosine diphosphate ribose) Identification of 2 -[l"-ribosyl-2"-(or 3"-)(l" -ribosyl)]adenosine-5, 5, 5 -ttis(phosphate) as a branch linkage. Proc Natl Acad Sci USA 1979 76 595-599. 

The events triggering the observed changes in the pattern of poly(ADP-ribose) modified proteins could result from an alteration in the structure of chromatin during myogenesis. Such alteration may affect the accessibility of nuclear acceptors and shift the equilibrium from modification of the 116 kD protein to that of differentiation associated acceptors of poly(ADP-ribose). Identification of the poly(ADP-ribose) modified acceptors will help elucidate their role in myogenesis. 

Reduction, alteration of nucleic acids by, 944-945 Relative abundance (RA), 242 Retinol. See Lipophilic vitamins, vitamin A Reversed phase (RP) TLC, 18 of dyes, 1026-1027 separation of peptides by, 440,441 solvents for, 25 of steroids, 976-977 Riboflavin (vitamin Ba), 1048 Ribonucleic acids, 956 Ribonucleosides, 956 Riboses, identification of, 937 Rifamycin, 476-478 Robotics, 381-382,384,959 Rolitetracycline, 468-473 Rotachrom (RPC instrumentation), 328-329 Rotation planar chromatography (RPC), 29-30, 172, 307, 324-325 applicability, 335... 

In as far as other analytical methods are concerned, many specific reactions have been elaborated for the quantitative determination of 2-deoxy aldoses. 2-Deoxy-D-ribose (2-deoxy-D-erythro-pentose), a compound which was recognized early as playing an important role in biological systems, has been of particular interest. Overend and Stacey (43) have given a critical review of the methods available until 1952 for the estimation of 2-deoxy pentoses. A recent summary of specific methods for the identification and quantitative estimation of the different classes of deoxy sugars has been prepared by Dische (13). 

Ribose and a-glucose are monosaccharides that occur in nature. The carbon atoms are numbered for identification purposes. 

Adamietz P, Rudolph A (1984) ADP-ribosylation of nuclear proteins in vivo. Identification of histone H2B as a major acceptor for mono- and poly(ADP-ribose) in dimethyl sulfate-treated hepatoma AH 7974 cells. J Biol Chem 259 6841-6846... 

Ferro A, Olivera B (1982) Poly(ADP-ribosylation) in vitro. J Biol Chem 257 7808-7813 Gagne JP, Hunter JM, Labrecque B, Chabot B, Poirier GG (2003) A proteomic approach to the identification of heterogeneous nuclear ribonucleoproteins as a new family of poly(ADP-ribose)-binding proteins. Biochem J 371 331-340... 

Li B, Navarro S, Kasahara N, Comai L (2004) Identification and biochemical characterization of a Werner s syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1. J Biol Chem 279 13659-13667... 

H. Redlich, W. Bruns, W. Francke, V. Schurig, T. L. Payne, and J. P. Vit6, Chiral building units from carbohydrates. XIII. Identification of the absolute configuration of emfo-brevicomin from Dendroclonus frontalis and synthesis of both enantiomers from D-ribose, Tetrahedron 43 2029 (1987). 

Table IV, which briefly summarizes the material described in previous sub-sections, shows the total of the different monosaccharide components identified in bacterial polysaccharides, and our present knowledge about their activated forms. It may be seen that identification of the activated forms has been achieved for only approximately half of the monosaccharides known to be involved. The most striking gap in the information available is the lack of data about the activated forms of D-ribose-derived monosaccharides and of most of the D-fructose-derived aldoses having configurations other than gluco, galacto, and manno.

Stelter L, von Sonntag C, Schulte-Frohlinde D (1975a) Radiation chemistry of DNA-model compounds VIII. Dephosphorylation products from reactions of OH radicals with ribose-5-phos-phate in aqueous solution. The effect of oxygen. Z Naturforsch 30b 609-615 Stelter L, von Sonntag C, Schulte-Frohlinde D (1975b) Radiation chemistry of DNA-model compounds, VII. On the formation of 5-deoxy-D-eryffrro-pentos-4-ulose and the identification of 12 further products from y-irradiated aqueous solutions of ribose-5-phosphate. Z Naturforsch 30b 656-657... 

Aksamit, R. and Koshland, D. E., Identification of the ribose binding protein as the receptor for ribose chemotaxis in Salmonella typhimurium, Biochemistry, 13, 4473, 1974. 

Using benzimidazole derivatives Gulland and various coworkers9 10-11 have recently restudied the problem of the identification of D-ribose in yeast nucleic acid. 

D-Ribopyranose tetraacetate56 has been used as a derivative for the identification of D-ribose14 and, more recently, Hardegger, Schreier and El Heweihi57 have prepared dimethyl, ethylene and dibenzyl thioacetals (mercaptals) of D-ribose for this purpose. According to Zinner58 the di-n-propyl and diisobutyl thioacetals are particularly suitable derivatives for the identification of ribose. 

While the melting points of the aniline glycosides, the so-called anilides, are too variable for identification purposes, the partially etherified sugars form anilides which have reliable melting points and such anilides are widely used for identification purposes. Sirupy 2,3,5-trimethyl-D-ribose, for instance, has been converted into a crystalline anilide,89 presumably aniline 2,3,5-trimethyl-D-ribofuranoside. An investigation of the configuration and properties of such derivatives would seem highly desirable. 

The use of alkyl and alkaryl thioacetals for the identification of ribose57,68 as well as for the preparation of ribose derivatives90,102,153 has already been mentioned. D-Ribose appears to react with thiols somewhat more rapidly than the majority of sugars, a fact which may in part be attributable to its greater solubility in hydrochloric acid.58... 

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. In Nature (London) 340 680-685 Laugwitz KL, Spicher K, Schultz G et al. (1994) Identification of receptor-activated G proteins selective immunoprecipitation of photolabeled G-protein a subunits. In Methods Enzymol. 237 283-294 Meyer T, Hilz H (1986) Production of anti-(ADP-ribose) antibodies with the aid of a dinucleotide-pyrophosphatase-resistent hapten and their application for the detection of mono(ADP-ribosyl)ated polypeptides. In Ear. J. Biochem. 155 157-165... 

Schieberle, P, Hofmann, T., Identification of the key odorants in processed ribose-cysteine Mad-lard mixtures by instrumental analysis and sensory studies. In Flavour Science. Recent Developments, 8th Weurman Flavour Research Symposium (Taylor, A.J., Mottram, D.S., eds.), Royal Society of Chemistry, Cambridge, pp 175-181, 1996... 

Kameshita, I., Matsuda, Z., Tanigushi, T., Shizuta, Y. (1984). Poly(ADP-ribose)synthetase. Separation and identification of three proteolytic fragments as the substrate-binding domain, the DNA binding domain, and the automodification domain. J. Biol. Chem. 259,4770-4776. 

Moss, J., Jacobson, M. K., Stanley, S. J. (1985). Reversibility of arginine-specific mono(ADP-ribo-syl)ation identification in erythrocytes of an ADP-ribose-L-arginine cleavage enzyme. Proc. Natl. Acad. Sci. USA 82, 5603-5607. 

Conrad, F., Hanne, A., Gaur, R.K. and Krupp, G. (1995). Enzymatic synthesis of 2 -modified nucleic acids identification of important phosphate and ribose moieties in RNase P substrates. Nucleic Acids Res. 23, 1845-1853. 

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