Ribonucleic acid 2-deoxy

Section 28 7 Nucleic acids are polynucleotides present m cells The carbohydrate component is D nbose m ribonucleic acid (RNA) and 2 deoxy d ribose m deoxyribonucleic acid (DNA)... 

Nucleic Acids. Phosphoms is an essential component of nucleic acids, polymers consisting of chains of nucleosides, a sugar plus a nitrogenous base, and joined by phosphate groups (43,44). In ribonucleic acid (RNA), the sugar is D-ribose in deoxyribonucleic acids (DNA), the sugar is 2-deoxy-D-ribose. 

In view of the difficulty of hydrolyzing the pyrimidine nucleosidic linkages, ribonucleic acids have been hydrolyzed to a mixture of purine bases and pyrimidine nucleotides which is then separated by paper chromatography.132, 163 164 This method has been employed extensively for the analysis of ribonucleic acids, and gives reproducible results,166 but it has not been used to any great extent for deoxyribonucleic acids, probably because, under these conditions of hydrolysis, they yield some pyrimidine deoxy-ribonucleoside diphosphates.166... 

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

FIGURE 7-1 Representative monosaccharides, (a) Two trioses, an aldose and a ketose. The carbonyl group in each is shaded, (b) Two common hexoses. (c) The pentose components of nucleic acids. D-Ribose is a component of ribonucleic acid (RNA), and 2-deoxy-o-ribose is a component of deoxyribonucleic acid (DNA). 

Pyrimidine natural products are particularly important (Scheme lb). The nucleic acids contain pyrimidine and purine bases ribonucleic acids (RNA) contain D-ribose and uracil, deoxyribonucleic acids (DNA) contain 2-deoxy-D-ribose and thymine and both types contain phosphate residues,... 

When presynaptic neurons use monoamine neurotransmitters, they manufacture not only the monoamine neurotransmitters themselves but also the enzymes for monoamine synthesis (Fig. 1—7), the receptors for monoamine reuptake and regulation (Fig. 1—8) and the synaptic vesicles loaded with monoamine neurotransmitter. They do this on receiving instructions from the "command center or headquarters, namely the cell nucleus containing the neuron s deoxy-ribonucleic acid (DNA). These activities occur in the cell body of the neuron, but then monoamine presynaptic neurons send all of these items to the presynaptic nerve terminals, which act as field offices for that neuron throughout the brain (Figs. 1—1 to 1—3, 1—7, 1—8). Neurotransmitter is thus packaged and stored in the presynaptic neuron in vesicles, like a loaded gun ready to fire. 

The term nucleoside was originally proposed by Levene and Jacobs in 1909 for the carbohydrate derivatives of purines (and, later, of pyrimidines) isolated from the alkaline hydrolyzates of yeast nucleic acid. The phosphate esters of nucleosides are the nucleotides, which, in polymerized forms, constitute the nucleic acids of all cells.2 The sugar moieties of nucleosides derived from the nucleic acids have been shown, thus far, to be either D-ribose or 2-deoxy-D-eri/fAro-pentose ( 2-deoxy-D-ribose ). The ribo-nucleosides are constituents of ribonucleic acids, which occur mainly in the cell cytoplasm whereas 2-deoxyribo -nucleosides are components of deoxypentonucleic acids, which are localized in the cell nucleus.3 The nucleic acids are not limited (in occurrence) to cellular components. They have also been found to be important constituents of plant and animal viruses. 

Analogous reactions have been carried out with uridine (CXI, b series) and with l-jS-D-ribofuranosylthymine (CXI, c series).62 The latter series provided an avenue to the synthesis of 5 -methylcytidine, a nucleoside which is probably identical with that detected by Dunn60 in the ribonucleic acid of wheat germ. The thiation process has also been applied to the synthesis of 5 -fluorocytidine and 2-deoxy-5 -fluorocytidine261 from 5 -fluoro-uridine and 2-deoxy-5 -fluorouridine,209,228 respectively.2618... 

All our descriptions of ribonucleosides, ribonucleotides, and ribonucleic acid also apply to the components of DNA. The principal difference between RNA and DNA is the presence of D-2-deoxyribose as the sugar in DNA instead of the D-ribose found in RNA. The prefix deoxy- means that an oxygen atom is missing, and the number 2 means it is missing from C2. 

In sugars, the hydroxyl group is replaced by a hydrogen to produce deoxy sugars (devoid of oxygen). The important deoxy sugar is 2-deoxy ribose that occurs in deoxy ribonucleic acid. Other important deoxy sugars are L-fucose and L. rhamnose. 

In deoxyribonucleic acid (DNA) the carbohydrate is 2-deoxy-D-ribose, while in ribonucleic acid (RNA) the carbohydrate residue is ribose. Three types of RNA were recognized, and they can be messenger RNA (mRNA), transfer RNA (tRNA), or ribosomal RNA (rRNA), which is the most abundant in cells. Values between 10 and 10 Dalton have been reported for the molecular weight of DNA, and the molecular weight is about 10 for rRNA, 10 for mRNA, and lOMor rRNA. The simplified structures of DNA and RNA are the following ... 

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