Rhodobacter sphaeroides strain

Fig. 25. Electron transfer pathways in the RC isolated from Rhodobacter Sphaeroides strain R-26. (BChl)2 is dimer of the bacterioehlorophyll, BPh is the bacteriopheophytin, Q, -ubiquinone. The electron transfer rates are for the native RC with ubiquinone —10 as Q,v at room temperature. The rates given in parenthesis were determined below 100 K [194-197]...

ABSTRACT The structure of the reaction center (RC)f om Rhodobacter sphaeroides strain R-26 has been refined to an R value of 21% at 2.8 A resolution. Progress in the determination of modified RCs is described. In particular, the structure of RCs with only I quinone and with bound herbicide are reported. 

The reaction centers were isolated from Rhodobacter sphaeroides strain R-26 whole cells according to a procedure similar to those of Feher (1971) and Clayton and Wang (1971). This was as follows The Rb, sphaeroides whole cells were diluted to an... 

M. Huber, E.L. Lous, R. A. Isaacson, G. Feher, D. Gaul, and C.C. Schenck, EPR and ENDOR studies of the oxidized donor in reaction centers of Rhodobacter sphaeroides strain R-26 and two heterodimer mutants in which histidine M202 or L173 was replaced by leucine, in "Reaction Centers of Photosynthetic Bacteria," M.-E. Michel-Beyerle, ed., Springer, Berlin (1990). 

Several bacterial species were used for preparing RCs and/or chromatophores Rhodobacter sphaeroides strains R26 and 2-4-1, Rhodospirillum rubrum strain G9. Light-... 

McAuley-Hecht, K. E., Fyfe, P. K., Ridge, J. P., Prince, S. M., Hunter, C. N., Isaacs, N. W., Cogdell, R. J., and Jones, M. R., 1998, Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides Monitoring the optical properties of the complex from bacterial cell to crystal. Biochemistry, 37 4740n4750. 

Figure 2 H2 production from the lactic acid fermentate of the C. reinhardtii biomass by various photosynthetic bacteria. The fermentate of C. reinhardtii biomass was diluted to give a lactic acid concentration of 30 mmol/1, inoculated with one of the five strain of photosynthetic bacteria (O, Rhodobacter sphaeroides A, Rhodobacter capsulata , Rhodospirillum rubrum 9, Rhodovulum sulfidophilus , Rhodobium marinum), and incubated under illumination of 330 W/m2 at 30°C.

Reiss-Husson F and Mantele W (1988) Spectroscopic characterization of reaction center crystals from the carotenoid-containing wild-type strain Rhodobacter sphaeroides Y. FEES Lett 239 78-82... 

The three dimensional structure of the reaction center (RC) from Rhodobacter sphaeroides R-26 and 2.4.1 has been reported at a resolution of 2.8 A and 3.0 A respectively (1-4). To improve the accuracy of these models we have futher refined the R-26 data using molecular dynamics methods. We have also collected diffraction data at higher resolution for RCs from the 2.4.1 strain. To relate the three dimensional structure to its function, we are performing various studies on RCs with modified structures (altered amino acid composition, cofactors removed, with herbicide bound). We describe the structure of RCs containing only the primary quinone and the structure of RCs with the herbicide, terbutryn, bound. Progress in crystallizing and determining the structures of other modified RCs is also reported. 

X-ray diffraction methods have provided the detailed structures of the reaction centers from two carotenoid-containing puiple photosynthetic bacterial species, Rhodopseudomonas viridis [1] and Rhodobacter sphaeroides wild type strain 2.4.1 [2]. The coordinates of these structures indicate that the reaction center-bound carotenoid is located in the M subunit, close ( 4A) to the accessory bacteriochlorophyll monomer on the M subunit side and -lO.SA edge-to-edge distance from the primary donor. These structures suggest an involvement of the M-side monomeric bacteriochlorophyll in triplet-triplet energy transfer, but there has been no direct experimental verification of this hypothesis. 

We recently came back to the determination of equilibrium constants and addressed the case of bacterial photosynthesis [3]. We studied electron transfer reactions between primary and secondary donors in the R26 mutant strain of Rhodobacter sphaeroides. The positive charges formed on P870 are first transferred to soluble cyt c2... 

Plasmids in photosynthetic bacteria have been isolated from Rhodospiri1lum rubrum. Rhodobacter sphaeroides and Rhodobacter cap-sulatus (1 5). The sizes of their plasmids ranges from 40 to 200 kilobases. Kuhl et al. (3) have isolated the identical plasmids from nine strains of R. rubrum. In addition, they have reported that the plasmid-less mutants show different colors from the wild strain and can not grow photosynthetically in the light (6). In order to elucidate the biological roles of the plasmids in photosynthetic bacteria, it is essential to determine their DNA sequences. The present study reports the restriction map and the partial nucleotide sequences of R. rubrum plasmid. 

Rhodobacter sphaeroides wild type and mutant strain GA and Rhodo-spirillum rubrum wild type and mutant strain G9 were grown in Sistroms medium in 250ml flasks at 50 W.m" and 30 C or in a fermentor at 200 W.m and 30 C. For the measurements cells from the exponential growth phase were centrifuged and resuspended in buffer (MOPS, pH 7.0) or fresh Sistrom medium (pH 6.8). 

Kien NB, Kong IS, Lee MG, Kim JK (2010) Coenzyme QIO production in a 150-1 reactor by a mutant strain of Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 37 521-529 Kim SW, KeasUng JD (2001) Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng 72 408 15... 

Johnson, E.T., Mtih, F., Nabedryk, E., Williams, J.C., Allen, J.P., et al. Electronic and vibronic coupling of the special pair of bacteriochlorophylls in photosynthetic reaction centers fi om wild-type and mutant strains of Rhodobacter sphaeroides. J. Phys. Chem. B 106, 11859-11869 (2002)... 

Frank, H. A., Aldema, M. A., Violette, C. A. and Parot, P. H., 1991, Low temperature polarized absorption microspectroscopy of single crystals of the reaction center from Rhodobacter sphaeroides wild type strain 2.4.1. Photochem. Photobiol. 54 151-155. 

Almost all the phototrophic bacteria strains used in hydrogen production are from the genera Rhodobacter (and the most used species is R. sphaeroides). Some species formerly designated as Rhodopseudomonas have been classified as Rhodobacter in recent classification criteria (information from Jun Miyake). Maybe some strains designated as Rhodopseudomonas and Rhodospirillum should be re-classified under new criteria, but in this column is presented the name suggested by the authors. Adaptation of strains to particular media or the selection of mutants, maybe is not enough to define new species. 

Wild type reaction centers from Rb. sphaeroides were isolated from the deletion strain complemented with the wild type genes. Reaction centers from Rhodobacter capsulatus were isolated as previously described. 

---