Rhodobacter sphaeroides wild type strain

X-ray diffraction methods have provided the detailed structures of the reaction centers from two carotenoid-containing puiple photosynthetic bacterial species, Rhodopseudomonas viridis [1] and Rhodobacter sphaeroides wild type strain 2.4.1 [2]. The coordinates of these structures indicate that the reaction center-bound carotenoid is located in the M subunit, close ( 4A) to the accessory bacteriochlorophyll monomer on the M subunit side and -lO.SA edge-to-edge distance from the primary donor. These structures suggest an involvement of the M-side monomeric bacteriochlorophyll in triplet-triplet energy transfer, but there has been no direct experimental verification of this hypothesis. 

Frank, H. A., Aldema, M. A., Violette, C. A. and Parot, P. H., 1991, Low temperature polarized absorption microspectroscopy of single crystals of the reaction center from Rhodobacter sphaeroides wild type strain 2.4.1. Photochem. Photobiol. 54 151-155. 

Rhodobacter sphaeroides wild type and mutant strain GA and Rhodo-spirillum rubrum wild type and mutant strain G9 were grown in Sistroms medium in 250ml flasks at 50 W.m" and 30 C or in a fermentor at 200 W.m and 30 C. For the measurements cells from the exponential growth phase were centrifuged and resuspended in buffer (MOPS, pH 7.0) or fresh Sistrom medium (pH 6.8). 

Reiss-Husson F and Mantele W (1988) Spectroscopic characterization of reaction center crystals from the carotenoid-containing wild-type strain Rhodobacter sphaeroides Y. FEES Lett 239 78-82... 

McAuley-Hecht, K. E., Fyfe, P. K., Ridge, J. P., Prince, S. M., Hunter, C. N., Isaacs, N. W., Cogdell, R. J., and Jones, M. R., 1998, Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides Monitoring the optical properties of the complex from bacterial cell to crystal. Biochemistry, 37 4740n4750. 

Johnson, E.T., Mtih, F., Nabedryk, E., Williams, J.C., Allen, J.P., et al. Electronic and vibronic coupling of the special pair of bacteriochlorophylls in photosynthetic reaction centers fi om wild-type and mutant strains of Rhodobacter sphaeroides. J. Phys. Chem. B 106, 11859-11869 (2002)... 

Wild type reaction centers from Rb. sphaeroides were isolated from the deletion strain complemented with the wild type genes. Reaction centers from Rhodobacter capsulatus were isolated as previously described. 

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