Wild-type genes

PSI] nomenclature SUP35 Wild-type gene encoding a subunit of the translation termination factor... 

Wild-type gene. The form of a gene (allele) normally found in nature. 

Figure 5. The a-L-fucosidase wild type gene organization (A) and preparation of the full-lenght mutant Ssa-fuc (B). The adjacent ORFs SSOl 1867 and SS03060 and the sequence of overlap are shown. The slippery sequence is underlined. The mutated bases are indicated with a star.

By this approach, positive clones are expected to arise from overexpression of wild-type genes conferring resistance or from frans-dominant mutations giving a mutant (resistant) phenotype (14). To identify loss-of-function recessive mutations the method should be reversed P-RES resistant clones should be transformed with a genomic library constructed with the DNA of the wild-type HB101, and clones with restored peptide susceptibility analyzed. 

There are conditions under which mildly deleterious mutations could contribute to protein evolution. If a second mutation occurs in a protein-coding gene that has experienced a deleterious mutation, it might compensate for the harmful effect of the first. If the doubly mutated gene were functionally equivalent to the wild-type gene, it would be fixed by genetic drift. 

Forward Mutation. A mutation that occurs in the wild-type gene to yield a mutant gene. 

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