Reverse-phase proteins

Millea, K.M., Kass, I.J., Cohen, S.A., Krull, I.S., Gebler, J.C., Berger, S.J. (2005). Evaluation of multidimensional (ion-exchange/reversed-phase) protein separations using linear and step gradients in the first dimension. J. Chromatogr. A 1079, 287-298. 

C.P. Paweletz, L. Charboneau, V.E. Bichsel, N.L. Simone, T. Chen, J.W. Gillespie, M.R. Emmert-Buck, M.J. Roth, E.F. Petricoin, and L.A. Liotta, Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene 20, 1981-1989 (2001). 

Antigen arrays, also described as reverse-phase protein arrays (Paweletz et al., 2001), involve the immobilization of proteins to serve as bait for various protein-protein interactions (Sreekumar and Chinnaiyan, 2002). For example, Joos et al. (2000) printed down various autoantigens present in sera with known associations with various autoimmune diseases such as Graves disease lupus connective tissue disease, and others. The group then screened various sera for the presence of autoantibodies. By immobilizing on the array a serial dilution series for each antigen, the titers for these antibodies could be determined. 

Reverse Phase Protein Microarrays and Their Utiiity in Drug Deveiopment... 

Key words Reverse phase protein microarrays, RPMA, Drug development, Drug discovery, Biomarkers,... 

The term reverse phase protein microarray was first coined in 2001 by Paweletz et al. (5) in the landmark paper which introduced the platform and demonstrated its utility for analyzing proteins derived from laser capture microdissected (LCM) prostate cancer. RPMAs have also been referred to by other names in the literature including lysate arrays, reverse phase lysate arrays, protein microarrays, and reverse phase protein arrays (6). A very nice historical perspective on the development of RPJMA technology is presented in a review by Mueller et al. (6). A detailed description of the methods for performing RPMA is beyond the scope of this chapter and would be redundant because there are... 

Espina, V. Reverse phase protein microarrays for monitoring biological responses... 

Gallagher, R.I. Reverse phase protein microarrays fluorometric and colorimetric detection... 

Wilson, B. Monitoring proteins and protein networks using reverse phase protein arrays... 

Espina, V. Phosphoprotein stability in clinical tissue and its relevance for reverse phase protein microarray technology... 

MueUer Reverse phase protein microarrays advance to use in clinical trials... 

Mueller C, Liotta LA, Espina V (2010) Reverse phase protein microarrays advance to use in clinical trials. Mol Oncol 4 461M81... 

Pierobon M, VanMeter AJ, Moroni N et al (2012) Reverse-phase protein microarrays. Methods Mol Biol 823 215-235... 

Espina V, Wulfkuhle J, Calvert VS et al (2008) Reverse phase protein microarrays for thera-nostics and patient-tailored therapy. Methods Mol Biol 441 113-128... 

Gallagher RI, Silvestri A, Petricoin EF et al (2011) Reverse phase protein microarrays fluorometric and colorimetric detection. Methods Mol Biol 723 275-301... 

Brase JC, Mannsperger H, Sultmann H et al (2011) Antibody-mediated signal amplification for reverse phase protein array-based protein quantification. Methods Mol Biol 785 55-64... 

Brase JC, Mannsperger H, Frohlich H et al (2010) Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification. Proteome Sci 8 36... 

Sheehan KM, Calvert VS, Kay EW et al (2005) Use of reverse phase protein microarrays and reference standard development for molecular network analysis of metastatic ovarian carcinoma. Mol Cell Proteomics 4 346-355... 

Lavezzari G, Lackner MR (2012) Monitoring phosphoproteomic response to targeted kinase inhibitors using reverse-phase protein microarrays. Methods Mol Biol 795 203-215... 

Martiny-Baron G, Haasen D, D Dorazio D et al (2011) Characterization of kinase inhibitors using reverse phase protein arrays. Methods Mol Biol 785 79-107... 

Zha H, Raffeld M, Charboneau L et al (2004) Similarities of prosurvival signals in Bcl-2-positive and Bcl-2-negative follicular lymphomas identified by reverse phase protein microarray. Lab Invest 84 235-244... 

Hennessy BT, Lu Y, Gonzalez-Angulo AM et al (2010) A technical assessment of the utility of reverse phase protein arrays for the study of the functional proteome in non-microdis-sected hiunan breast cancers. Clin Proteomics 6 129-151... 

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