Reversed-phase chromatography membrane proteins

Here again, not all chromatographic setups are usable for any proteomics question. The use of protein reverse phase chromatography, which has been advocated for plasma proteomics (Moritz et al. 2005), precludes in turn the use of any detergent of any type. This prevents the use of this chromatographic setup in most subcellular proteomics experiments, where detergents must be used to solubilize the membrane limiting the subcellular compartments. 

Tennikova, T. B., and Svec, F. (1993). High-performance membrane chromatography Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reverse-phase modes. J. Chromatogr. 646, 279-288. 

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