Restriction site analysis

A North American origin for the silverswords clearly emerged from the application of DNA-sequence techniques to members of the alliance and representative Californian species (Baldwin et al., 1990, 1991). The conclusions from these studies, based on chloroplast restriction site analysis were subsequently corroborated by ITS nuclear ribosomal DNA studies. A comprehensive review of these studies can be found in Carlquist et al. (2003). 

Schilling, E. E., Panero, J. L. and Eliasson, U. A. 1994. Evidence from chloroplast DNA restriction site analysis on the relationships of Scalesia (Asteraceae Hehantheae). Amer. J. Bot. 81 248-254. 

Saiki RK, Schare S, Faloona F Muelis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Science 1985 Dec 20, 23o(4732) i35o-i354-... 

Previously described methods for plant DNA extraction (e.g., see Hillis et al.16 and references therein) have usually either required CsCl purification or resulted in a final DNA product that often contained other contaminating substances. For restriction site analysis of chloroplast or mitochondrial genomes, where usually less than 0.5 fig of DNA per individual is required, any procedure was appropriate and practical. However, for minisatellite restriction enzyme analyses, typically 5-10 jtg of DNA is required per individual per trial. For such analyses, obtaining enough DNA from an individual for several trials using CsCl is costly and time-consuming ... 

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