Resolution sites

The resolvases act on supercoiled cointegrated DNA molecules that contain two directly repeated res sites to produce two singly linked circles (which are still supercoiled) each containing one res site as shown in Fig. 27-32. The two res (resolution) sites within the transposons are aligned, the open circle of DNA shown at the upper left being folded as shown in the lower part of the drawing. The DNA substrate is not knotted. However, after recombination it is catentated and will require action of a topoisomerase to separate... 

Chiorini JA, Afione S, Kotin RM. Adeno-associated vims (AAV) type 5 Rep protein cleaves a unique terminal resolution site compared with other AAV serotypes. J Virol 1999 73 4293 298. 

The paper presents the results from systematic comparisons of contrast and resolution obtained with different types of radiation sources on steel thicknesses from 5 to 40 mm. These results have been taken into account with the definitions of the European standard for radiographic inspection of weldments (EN 1435) that is approved since 1997. Conclusions from practical investigations on pipe line sites, in petrochcemical plants and in nuclear power stations will be discussed as well. Furthermore, the presentation will stipulate a variety of advantages obtained from the new source in terras of coUimation and radiation protection. 

The dependence of chiral recognition on the formation of the diastereomeric complex imposes constraints on the proximity of the metal binding sites, usually either an hydroxy or an amine a to a carboxyHc acid, in the analyte. Principal advantages of this technique include the abiHty to assign configuration in the absence of standards, enantioresolve non aromatic analytes, use aqueous mobile phases, acquire a stationary phase with the opposite enantioselectivity, and predict the likelihood of successful chiral resolution for a given analyte based on a weU-understood chiral recognition mechanism. 

Instrumental Interfaces. The basic objective for any coupling between a gas chromatograph (gc) and a mass spectrometer (ms) is to reduce the atmospheric operating pressure of the gc effluent to the operating pressure in the ms which is about 10 kPa (10 torr). Essential interface features include the capability to transmit the maximum amount of sample from the gc without losses from condensation or active sites promoting decomposition no restrictions or compromises placed on either the ms or the gc with regard to resolution of the components and reliability. The interface should also be mechanically simple and as low in cost as possible. 

Figure 4.8 The active site in all a/p barrels is in a pocket formed by the loop regions that connect the carboxy ends of the p strands with the adjacent a helices, as shown schematically in (a), where only two such loops are shown, (b) A view from the top of the barrel of the active site of the enzyme RuBisCo (ribulose bisphosphate carboxylase), which is involved in CO2 fixation in plants. A substrate analog (red) binds across the barrel with the two phosphate groups, PI and P2, on opposite sides of the pocket. A number of charged side chains (blue) from different loops as welt as a Mg ion (yellow) form the substrate-binding site and provide catalytic groups. The structure of this 500 kD enzyme was determined to 2.4 A resolution in the laboratory of Carl Branden, in Uppsala, Sweden. (Adapted from an original drawing provided by Bo Furugren.)...

Knight, S., Andersson, I., Branden, C.-I. Crystallographic analysis of ribulose-l,5-bisphosphate carboxylase from spinach at 2.4 A resolution. Subunit interactions and active site. /. Mol. Biol. 215 113-160,... 

A continuous lipidic cubic phase is obtained by mixing a long-chain lipid such as monoolein with a small amount of water. The result is a highly viscous state where the lipids are packed in curved continuous bilayers extending in three dimensions and which are interpenetrated by communicating aqueous channels. Crystallization of incorporated proteins starts inside the lipid phase and growth is achieved by lateral diffusion of the protein molecules to the nucleation sites. This system has recently been used to obtain three-dimensional crystals 20 x 20 x 8 pm in size of the membrane protein bacteriorhodopsin, which diffracted to 2 A resolution using a microfocus beam at the European Synchrotron Radiation Facility. 

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