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Renal in vitro models

STEM CELL-DERIVED RENAL CELLS AND PREDICTIVE RENAL IN VITRO MODELS... [Pg.365]

A race on the generation of stem cell-derived renal cells started in 2013. In January 2013 a protocol for the differentiation of hiPSC or hESC into IM was published (Mae et al., 2013). Odd-skipped related (OSR)l was used as the main IM marker, and up to 90% OSRO cells were obtained. More differentiated renal cell types were only obtained at low frequency, which was not sufficient for use of these cells in any application, including in vitro toxicology (Mae et al., 2013). However, briefly afterward the first protocol for the differentiation of human pluripotent stem cells (hPSC) into mature renal cells was published (Narayanan et al., 2013). This protocol was based on hESC, and the differentiated hESC-derived cells exhibited many features of HPTC and were therefore called HPTC-like cells. The hESC-derived HPTC-like cells were then directly used for the development of the first stem cell-based renal in vitro model for the prediction of DIN (Li et al., 2014). (This in vitro model will be described in more detail in the following.) Later in 2013 the race on the generation of stem cell-derived renal cells continued and various alternative protocols were developed (Lam et al., 2014 Xia et al., 2013 Kang and Han, 2014 Taguchi et al., 2014 Takasato et al., 2014) (Fig. 23.1). [Pg.369]

In Vitro Models that have been Tested with Higher Numbers of Compounds and the First Predictive Renal In Vitro Model... [Pg.376]

Six renal in vitro models have been tested with more than 10 compounds (Duff et al., 2002 Wu et al., 2009 Lin and Will, 2012 Li et al., 2013, 2014 Kandasamy et al., 2015) and in four cases performance metrics and predictivity were... [Pg.376]

Prediction of Renal PT Toxicity with HPTC HPTC were used for the development of the first predictive renal in vitro model (Li et al., 2013) (Table 23.1). Three batches of HPTC derived from different donors were screened with 41 compounds. Human (HK-2) and porcine (LLC-PKl) immortalized renal proximal tubular cell lines were nsed for comparison. All of the 41 compounds were either widely used drugs or environmental toxicants and indnstrial chemicals with well-characterized effects on human kidneys. Thus, the in vitro results could be directly compared to human clinical data. The compounds were classified into three groups (1) nephrotoxicants that are directly toxic for PTC in human kidneys, (2) nephrotoxicants that damage the kidney in different ways and are not directly toxic for PTC in humans, and (3) compounds that are not nephrotoxic in humans. [Pg.377]


See other pages where Renal in vitro models is mentioned: [Pg.365]    [Pg.366]    [Pg.367]    [Pg.367]    [Pg.373]    [Pg.373]    [Pg.374]    [Pg.376]    [Pg.376]   
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