Remazol Brilliant Blue

Fig. 1. Blue reactive dyes from antliiaquinone. Also see Table 6. Reactive Blue 5 [16823-51 -1] (Cl 61210) (19), Reactive Blue 4 [13324-20-4] (Cl 61205) (20), eg, Piocion Blue MX-R, Reactive Blue 19 [2580-78-1] (Cl 61200) (21), eg, Remazol Brilliant Blue R.

Eichlerova I, Homolka L, Lisa L, Nerud F (2005) Orange G and Remazol Brilliant Blue R decolorization by white rot fungi Dichomitus squalens Ischnoderma resinosum and Pleurotus calyptratus. Chemosphere 60 398 -04... 

Deveci T, Unyayar A, Mazmanci MA (2004) Production of Remazol Brilliant Blue R decolourising oxygenase from the culture filtrate of Funalia trogii ATCC 200800. J Mol Catal B Enzym 30 25-32... 

G. Palmieri, G. Cennamo and G. Sannia, Remazol Brilliant Blue R decolorisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system. Enz. Microbiol. Technol., 36 (2005) 17-24. 

Direct visualization of the fluxes involved was achieved using a blue-labelled PVP obtained by coupling PVP to remazol brilliant blue 46). When a free boundary was formed in a Tiselius diffusion cell, a striking series of events was seen. After an... 

Overall crude cellulase activity was measured using Remazol brilliant blue acid-swollen cellulose (cellulose-azure, Calbiochem.) (22). The assay consists of combining 40 mg cellulose azure, 1.0 mL of lOOmM sodium citrate buffer (pH 4.8), and 1.0 mL of enzyme solution and... 

High-molecular-weight solid substrates such as hide powder azure, and cellulose, chitin, and agar stained with remazol brilliant blue R have been used in enzyme assays to investigate activities in extracts from sediments (Reichardt, 1986). This technique is one of the few means of examining the particle — dissolved transition in relationship to sedimentary enzyme activity, but the necessity of extracting enzymes from sediments complicates interpretation of the results, because extraction efficiency, as well as the... 

SYNS CAVALITE BRILLIANT BLUE R C.I. 61200 C.I. REACTIVE BLUE 19 C.I. REACTIVE BLUE 19, DISODIUM SALT REACTIVE BLUE 19 REMALAN BRILLIANT BLUE R REMAZOL BRILLIANT BLUE R... 

To observe the movements of the biopolymers into and retention in the vegetable cells, the biopolymers were modified into colored compounds suitable for use as markers in microscopic examinations. The biopolymers were reacted with Remazol brilliant blue R salt according to Rinderknecht et al. (32) with modifications. The reaction created covalent bonds between the blue dye and hydroxyl groups of the biopolymers. The dye-biopolymyer complexes have the same physical properties as natural biopolymers. These types of dyes have been used for cellulose materials (33) and galacto-D-Mannan (34). 

Rinderknecht et al. (14) designed a liquid assay for measuring a-amylase activity using amylose that had been covalently linked to a dye, Remazol Brilliant Blue R (RBB). In principle, this dye can be coupled to every soluble polysaccharide. RBB was coupled to... 

Mannans. — D-Galacto-D-mannan dyed with Remazol Brilliant Blue has been used in a simple assay procedure for D-mannanase. The procedure is quantitative, sensitive, and highly specific. 

Chromogenlc substrates Ostazln Brilliant Red hydroxyethylcellulose (OBR-HEC), a soluble covalently dyed cellulose derivative, a substrate specific for endo-l,4-3-glucanases (cellulases) [4] Remazol Brilliant Blue xylan (RBB-X), a soluble covalently dyed beechwood 4-0-methyl-D-glucurono-D-xylan, a specific substrate for endo-l,4-B-xylanases [4]. Both substrates are available on request from Chemapol Ltd., Praha, Czechoslovakia. 

The specific activities of purified preparations of human parotid and pancreatic amylases towards Amylose Azure, Remazol Brilliant Blue starch, phosphate-derivatized starch, and cross-linked starch have been measured. The activities varied with the physical state of the substrate the parotid enzyme exhibited a higher specific activity towards all soluble starches, whereas the pancreatic amylase was more active towards insoluble chromogenic starches. Variations in the enzyme-substrate interactions support the view that there is a configurational difference between the amylases this may be useful in determining the proportions of these amylases in normal and pathological body fluids. 

A dyed derivative of starch (Remazol Brilliant Blue starch), phosphate-derivatized starch, and cross-linked starch have been compared as substrates for a-amylases. The different susceptibilities of the substrates to hydrolysis was used to ascertain the configurational requirements of the enzymes. 

Celluloses dyed with Remazol Brilliant Blue R, etc., have been used as substrates in determining the solubilizing activities of cellulase complexes. ... 

A Blue Starch-agar plate, Phadebas Blue starch, - and Remazol Brilliant Blue starch have been used in the detection of amylase activity. 

FIGURE 8.1 Ganoderma lucidum laccase activity after SDS-PAGE. Gel was incubated in Remazol Brilliant Blue R solution and decolorization of dye is seen surrounding the laccase band (arrow, lane 2). Molecular weight markers are in lane 1. (Adapted with permission from Ref. [57]. Copyright 2007, Elsevier.)... 

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