Cellulose acid-swollen

Cellobiohydrolase I (CBH I, 1,4-jS-D-glucan-cellobiohydrolase, E.C. 3.2.1.91) is the main protein (ca. 60%) of the cellulase complex produced by T. reesei strains. CBH I hydrolyses crystalline cellulose, acid swollen cellulose and 4-methylumbelliferyl-cellodex-trins by cleaving off the terminal cellobiose unit from the non reducing end of the chain. It operates with retention of configuration in the reaction products 19,20. The abundance of this enzyme and its stability has facihtated its purification to homogeneity... 

Figure 10. Product distribution from the incubation of enzymes (produced by T. reesei QM 9414 in response to sophorose) with phosphoric-acid swollen cellulose (PSC). Incubation time in this figure indicates the length of incubation of T. reesei mycelia in the presence of ImM sophorose. Enzymes produced at those times were incubated with PSC for 15 hr, and the products analyzed by HPLC (18/...

Overall crude cellulase activity was measured using Remazol brilliant blue acid-swollen cellulose (cellulose-azure, Calbiochem.) (22). The assay consists of combining 40 mg cellulose azure, 1.0 mL of lOOmM sodium citrate buffer (pH 4.8), and 1.0 mL of enzyme solution and... 

Total cellulase complex 1.5% oxgall + Phosfon D acid swollen cellulose... 

B-glucosidase of C. gilvus. Cellobiose rather than glucose accumulated when C. gilvus ATCC 13127 was cultivated in a medium containing acid swollen cellulose... 

PMOs are supposed to be active only on (semi)ctystalline polysaccharides and not on short soluble oligosaccharides (DP 2-5). Harris et al. tried a whole variety of substrates and concluded that mixed substrates like PCS (pre-treated corn stover) generate higher activities than pure cellulose substrates like Avicel (microcrystalline cellulose) or PASC (phosphoric acid swollen cellulose).However, hemicellulose substrates do not tend to be more efficiently degraded by PMO addition. The first successful experiments with real pre-treated biomass, namely pre-treated spruce, were recently performed. ... 

Endoglucanases hydrolyze 3-l,4-glycosidic linkages randomly. They do not attack cellobiose but hydrolyze cellodextrins, phosphoric-acid swollen cellulose and substituted celluloses such as carboxymethyl (CM)- and hydroxyethyl (HE)-cellulose. Trichoderma seems to secrete a number of respective enzymes into the medium, of which two (EG I, EG III) have been the subject of a more detailed investigation. It should be noted that some confusion has arisen in the past from the fact that EG III was termed EG II by several authors from the US. However, this has been overcome now. 

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