Recombinant DNA principles

Section IV describes the structures and functions of the nucleotides and nucleic acids, and covers many major topics such as DNA replication and repair, RNA synthesis and modification, and protein synthesis. It also discusses new findings on how genes are regulated and presents the principles of recombinant DNA technology. 

Glick B.R Pasternak J.J. (1994) Molecular Biotechnology Principles and Applications of Recombinant DNA. Washington, D.C. American Society for Microbiology. 

Click BR, Pasternak JJ, In Molecular Biotechnology Principles And Applications of Recombinant DNA (Eds Click BR, Pasternak JJ), pp. 145-169. American Society for Microbiology, Washington DC, 1998. 

If biocatalysis is so attractive, why was it not widely used in the past The answer is that only recent advances in biotechnology have made it possible. First, the availability of numerous whole-genome sequences has dramatically increased the number of potentially available enzymes. Second, in vitro evolution has enabled the manipulation of enzymes such that they exhibit the desired properties substrate specificity, activity, stability, and pH profile [42]. Third, recombinant DNA techniques have made it, in principle, possible to produce virtually any enzyme for a commercially acceptable price. Fourth, the cost-effective techniques that have now been developed for the immobilization of enzymes afford improved operational stability and enable their facile recovery and recycling [43]. 

The principles that govern the delivery of recombinant DNA in clonable form to a host cell, and its subsequent amplification in the host, are well illustrated by considering three popular cloning vectors commonly used in experiments with E. coli—plasmids, bacteriophages, and bacterial artificial chromosomes—and a vector used to clone large DNA segments in yeast. 

L. Perez-Pons and E. Querol, Biochem. Educ. 24, 54-56 (1996). A laboratory experiment illustrating basic principles of DNA cloning and molecular biology techniques. J. Sambrook, E. Fritsch, and T. Maniatis, Molecular Cloning, A Laboratory Manual, 2nd ed. (1989), Cold Spring Harbor Laboratory (Cold Spring Harbor, NY), Vols. I, II, and III, pp. 1.21-1.52. Techniques in recombinant DNA. 

Experiment 14 introduced students to some principles and techniques involved in recombinant DNA research. Specifically, the experiment outlined the replication, isolation, and analysis of bacterial plasmid vehicles for molecular cloning experiments. This experiment describes another tool that is essential in hybrid plasmid construction and analysis-restriction enzyme action. The procedures introduced here have also found widespread use in the analysis and characterization of all DNA molecules. 

Glick BR, Pasternak JJ (1994) Molecular biotechnology principles and applications of recombinant DNA. ASM Press, Washington, DC... 

After many years, chemical reaction engineering has developed a paradigm classic papers that are universally admired, basic assumptions and analysis, successful applications of principles to particular problems, and standard textbooks and curricula that are generally accepted. Chemical reaction engineering is not yet completely matured and thus has not been reduced to restatements of old results and remeasurements with greater accuracy. The innovation processes continue to develop. New needs of society, such as synthetic fuels, and new technical opportunities, such as recombinant DNA, will keep this subject vigorous for many years to come. 

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