Recipient spleen

Starting with the second week of nevirapine treatment, progressive infiltration of T cells into the rat skin dermis was observed. To assess the specific role of the T cell populations in triggering the onset of skin rash, Shenton et al. transferred splenocyte T cells from rechallenged rats into naive recipients. Spleen CD4 T and CD8 T cells were isolated from the nevirapine-rechallenged rats, purified, and intravenously injected into naive recipients, which were then started on a full (150 mg/kg/day) nevirapine dose (Shenton et al. 2005). Recipients of CD4 T cells developed skin rash 9 days later, while CDS" T cell recipients behaved as nevirapine naive rats, only developing red ears by day 7 and skin rash by day 21... 

Figure 1. Experimental Set up. Irradiated female mice were transplanted with retrovirally transduced BM cells from male donors (6 mice per vector). After 6 months of monitoring a secondary BMT took place. Spleen cells were used for the analysis of retroviral vector insertion sites (RVIS) by means of Ligation Mediated PCR (LM PCR) °. After another 6 months follow up spleen cells of the secondary recipients were again analysed for RVIS via LM-PCR.

Figure 5. Detection of male MSC by Y-PCR. Y chromosome PCR at 24 h after MSC infusion from a male donor into female recipients with AKl. The only organ positive for Y chromosome DNA was the lung (lane 2). Kidney cortex and medulla as well as liver and spleen from 2 animals were negative (lanes 3-11). Lane designation A-female DNA B-male DNA 2 lung 3-kidney cortex 4-kidney medulla 5-liver 6-spleen 7-wound scar 8-lung 9-bone marrow 10-liver 11-spleen.

Table 5. Spleen colony-forming units (CFUs) detected in irradiated recipient mice after injection of bone marrow harvested from mice injected with free ADR or HPMA copolymer ADR conjugates (from [177] with permission)...

Importantly, Braun and coworkers [15] showed that spleen cells from the tolerant mice, when transferred to normal recipient mice, inhibited the SEB-induced IL-2 and IFN-y response in the recipients. This result formally demonstrated that the SEB-specific tolerance in mice exposed repeatedly to SEB is dominant and mediated by suppression. This inhibition could be reversed by in vivo treatment of recipient mice with neutralizing anti-IL-10 antibodies, suggesting that IL-10-secreting cells in the transferred population mediated the suppression. The... 

Levy EM, Bennett M, Kumar V, et al. 1980. Adoptive transfer of spleen cells from mice treated with radioactive strontium Suppressor cells, natural killer cells, and "hybrid resistance" in recipient mice. J Immunol 124(2) 611-618. 

Briles and Krause carried out adoptive transfers using 10 spleen cells from SWR/J mice that had produced antibody of restricted heterogeneity to group A streptococcal carbohydrate (118). Recipient mice produced antibodies that were identical to those of the donor by the criteria of electrophoretic mobility and idiotype. 

Figure 5.8 Adoptive immunization experiment production of SE agglutinins after transfer of pre-immunized spleen cells of High or Low responder into normal recipients of the opposite line. Donor immunization 5 x 10 SE intravenously 14 and 4 days before transfer. Transfer one spleen equivalent per recipient intraperitoneally. From Biozzi et al (1971). In Progress in Immunology, p. 529, by courtesy of Academic Press, New York.

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