Reagents and supplies

2 Reagents and supplies Acetonitrile, reagent grade and HPLC grade 

Distilled water (prepared with a Yamato WG-75 system or HPLC grade) 

Weigh 20 g of the plant sample into an Erlenmeyer flask and add 40 mL of 1 N HCl and 160 mL of acetonitrile. Shake the flask for 30 min at 300 strokes min using a shaker. Filter the aqueous acetonitrile extract through a No. 4 Kiriyama funnel filter paper. Wash the residue on the filter with 100 mL of acetonitrile. Combine the filtrates and remove acetonitrile with a rotary evaporator. Transfer the residue with 20 mL of saturated aqueous sodium chloride solution into a separatory funnel, extract the solution with 3 x 30 mL of n-hexane-ethyl acetate (9 1, v/v), and collect the organic phase in a flask. Dry with anhydrous sodium sulfate and remove the combined organic phase with a rotary evaporator. Transfer the residue into the Eppendorf tube with a 

The air-dried soils (50 g) are processed similarly to the plant materials. 

Adjust to pH 2 by addition of concentrated HCl to 500 mL of water sample. Apply this acidic water to the Bond Elut LRC Cig (500-mg) cartridge column. Pass the elution solvent (2 mL of acetonitrile) through the cartridge and collect in a test-tube. Concentrate the eluate under an N2 gas flow at about 40 °C. 

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Most mechanical hazards in the laboratory can be avoided by good planning. There must be no obstructions in traffic areas, particularly those needed for rapid evacuation in case of emergency. Wide hallways may look like good parking places for movable equipment not currently in use. They may also seem convenient for storage of reagents and supplies that temporarily... 

An additional phase of method feasibility that is often overlooked is an assessment of method items that may not have an equivalent replacement. In HPLC, an obvious item that falls under this category is the HPLC column. Other items, however, such as the actual instruments, equipment, reagents, and supplies used, must be evaluated during feasibility. It is important that... 

Variability experienced by the same analyst using the same instrumentation, reagents, and supplies in the same laboratory over a short time interval. 

Reagents and supplies for SDS-PAGE (see Experiment 4) Glass or plastic plates for casting SDS-polyacrylamide gel with a 10-well comb Plastic wrap... 

Reagents and supplies to perform SDS-PAGE—See Experiment 4. We suggest using a 12 or 15% polyacrylamide gel for this experiment. 

Comments Complete line of brand name and non-brand name laboratory equipment, accessories, reagents and supplies. Serving domestic and overseas industry and institutional research. 

As presented in the two previous subsections, several viable options exist for high throughput bioanalysis. It is interesting to note that an issue seldom discussed is the relative cost of the reagents and supplies associated with the various methods. For example, the issue of high mobile-phase consumption/waste-stream production in TFC may be a concern. A similar situation occurs with... 

This table illustrates one of the major impediments to the rapid assimilation of immunochemical technology into pesticide residue analysis labs. Because of the amount and variety of work involved, new method development costs may be high when compared to routine chromatographic methods. However, the low cost per run allows for rapid recovery of the initial investment with sufficiently high sample loads. For example, the cost of reagents and supplies for an ELISA for diflubenzuron was estimated to be 0.20/sample as compared with 4 for HPLC or 11 for GC (35). In addition to the lower reagent and supply costs, the major economic advantage of immunoassay is the dramatic decrease in labor costs. 

The cleanliness of equipment and reagents used in the test is of paramoimt concern. Mercury is a ubiquitous contaminant that can show up in suiprising ways, especially at the range of concentrations in the samples. An example of potential contamination is the mercury compounds that may be used as preservatives in certain phosphate buffer solutions. The analyst must be continuously vigilant when choosing reagents and supplies. 

Geneco s first synthesizers and sequlnators are not expected to be available until mld-1982, but other Instruments, reagents, and supplies will soon follow. 

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