Reagent handling and storage

The OPUS system has dry reagents impregnated in a coated multilayer film chip encased within a plastic bar coded test module. No external or supplementary reagents are required. A serum or plasma specimen is applied to the test module by the instrument and the fluorescence intensity from the module is measured after an assay-specific time interval. 

Some systems use reagents or antibodies that have been immobilized in a reaction coil or chamber to repetitively carry out a chemical reaction or a separation of art analyte contained in ahquots of successive specimens. Other systems use enzymes immobhized on membranes that are coupled to sensing electrodes. The reaction products are then measured by the sensing device. Only a buffer is required as a diluent and wash solution, and thus the membrane has an extended life of approximately several months. Some 

SPREADING LAYER REAGENT LAYER SEMI PERMEABLE LAYER 

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In order to achieve optimal performance from reagents used in immunohistochemistry, it is imperative to observe basic rules for their handling and storage. If properly maintained, most reagents will remain stable for months or even years. Recommendations given by the manufacturer on specification sheets and on vial labels should always be heeded. 

Several organometallic acetylene compounds are prepared by the replacement of acetylenic Grignard reagents or alkalimetal acetylides with metal halides (MX) where M is B, AI, Si, Ge, Sn, P, As, Sb, Cu, or Ag [128-131]. They are also synthesized by metallation of metal halides and R3AI or R2Hg (see Table 4, entries 40 and 41). Metal acetylides of the type (RC C)3B or (RC C)3A1 are unstable and are isolated as complex with amines or ethers. Note that many of the precious metal acetylides are extremely explosive, and the appropriate precautions should be taken for their handling and storage. 

Availability and choice of instrumentation and how use of different equipment could affect assay performance need to be discussed. Definitions of experimental and storage conditions, reagent sources and quality, and lab personnel experiences should be discussed early on. This also applies to the process of assigning expiry dates, assessment of reagent stability, reagent handling, and shipping processes. 

Consistent stability of the PEG chain and the linker under various conditions of handling and storage, which may be affected by trace impurities in the PEG reagent, is more difficult to address from batch to batch. However, this aspect, if not monitored properly, could cause signihcant problems in some API batches either in failing to meet product specihcations and/or in reduced efficacy. 

Solutions of butyllithium, and other lithium reagents, may be purchased from Aldrich. See Section 4.2.47, p. 442 for notes on the storage, handling and analysis of alkyllithium reagents. 

Handling, Storage, and Precautions the reagent is sensitive to air and should be handled and stored under argon or nitrogen. 

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