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Raman spectroscopy single cell analysis

Zheng F, Qin YJ, Chen K (2007) Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer. J Biomed Opt 12(3) 034002 Chan JW et al (2008) Nondestmctive identification of iudividual leukemia cells by laser trapping Raman spectroscopy. Anal Chem 80(6) 2180-2187... [Pg.528]

Wood et al. have combined acoustic levitation and Raman spectroscopy with the intention of developing a field tool for environmental monitoring of algal blooms and nutrient availability [62]. Heraud discussed the most appropriate spectral preprocessing techniques for analysis of Raman spectra of single microalgal cells and developed a method to predict the nutrient status of those cells from in vivo spectra [63,64]. [Pg.214]

The vast identification potential of micro-Raman spectroscopy combined with the aforementioned sophisticated chemometric analysis methods is corroborated within various works In one study a total of 2257 Raman spectra of single cells were used to differentiate among 20 strains belonging to 9 different species. Here, a recognition rate of 89.2% for strains using the SVM technique could be achieved, albeit these bacteria were grown under different conditions (cultivation time medium, and temperature). [45]... [Pg.450]

Harz M, Kiehntopf M, Stockel S, Rosch P, Deufel T, Popp J (2008) Analysis of single blood cells for CSF diagnostics via a combination of fluorescence staining and micro-Raman spectroscopy. Analyst 133 1416-1423... [Pg.444]

Raman spectroscopy is equally suitable for the analysis of gases, liquids, fibres, single crystals, surface features, etc. Intact measurements permit one to investigate the native molecular structure in biopolymers, living and other systems. It permits studies of eye lenses, the end processes of muscle contraction, components of living cells, and of ancient manuscripts and art objects, etc. The crystallinity of polymeric materials and orientation effects in fibres, monitored by FT-Raman spectra, could be very useful in technological control and in forensic science. [Pg.13]


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