Radiolabelling biodistribution

This first strategy was used by Setton and coworkers to trigger the in situ formation of an ELP-based dmg depot for sustained release. Biodistribution studies of radiolabeled LLPs with a below body temperature were performed after intra-articular injection in rats [94]. Later, dmg depots of ELPs with covalently attached immunomodulator therapeutics [95] and anti-TNLa therapeutics [96] were created. Respectively, ELP[V-120] and ELP[V-60] biopolymers with a transition below body temperature were used in these studies. 

The same group reported the simultaneous radiolabeling (with DOTA-anchored 4Cu) and fluorescence studies, coupled with biodistribution in vivo and in vitro (92). It is believed that appropriately functionalized SWNTs can efficiently reach tumor tissues in mice with no apparent toxicity (159). Furthermore, water-solubilised carbon nanotubes are nontoxic when taken up by cells even in high concentration (92). These studies have been complemented by the recent PET imaging of water-soluble 86Y labelled carbon nanotubes in vivo (mice) (160,161), to explore the potential usefulness of carbon nanocarriers as scaffolds for drug delivery. The tissue biodistribution and pharmacokinetics of model DOTA functionalized nanotubes have been explored in vivo (mouse model). MicroPET images indicated accumulation of activity mainly in the kidney, liver, spleen, and to a much less... 

Preclinical studies should address the potential toxicity due to inappropriate release of the conjugated toxin. Preclinical toxicology of monoclonal antibodies may not require extensive animal studies but should be examined for cross-reactivity with antigenic epitopes present on normal cells in vitro and for the presence of human or rodent vimses. Early clinical trial should involve biodistribution studies with radiolabelled material. 

Rudolph AS, Klipper RW, Goins B, et al. In vivo biodistribution of a radiolabeled blood substitute Tc-labeled liposome-encapsulated hemoglobin in an anesthetized rabbit. Proc Natl Acad Sci USA 1991 88 10976. 

Goins B, Phillips WT. Radiolabelled liposomes for imaging and biodistribution studies. In Torchilin VP, Weissig V, eds. Liposomes A Practical Approach. Oxford, U.K. Oxford University Press, 2003 319. 

Awasthi VD, Goins B, Klipper R, Phillips WT. Dual radiolabeled liposomes biodistribution studies and localization of focal sites of infection in rats. Nucl Med Biol 1998 25 155. 

Preliminary information gained on the in vivo biodistribution of double-radiolabeled nanoparticles recorded in rabbits nicely confirmed the in vitro test carried out on a primary cell line of rat hepatocytes. 

E = 137keV). The accompanying emission of 7-radiation can be used for scintigraphic imaging but also makes patient isolation necessary. The different half-lifes and /3 -energies allow individual therapeutic demands such as the pharmacokinetics of the tracer molecule, the linear energy transfer of the nuclides or the biodistribution and clearance of the radiolabeled drug to be met. The principles of the application of radioactive materials for therapy are summarized in an excellent review. ... 

Chemically modified allergen (allergoids) are frequently used for immunotherapy. We have comparatively evaluated the biodistribution of native radiolabelled Par j 1 and the corresponding monomeric carbamylated allergoid (monoid) [48]. 

Pimm, M. V., Durrant, L. G., and Baldwin, R. W. Influence of circulating antigen on the biodistribution and tumor localization of radiolabelled monoclonal antibody in a human tumor Nude mouse xenograft model. Eur. J. Cancer. Clin. Oncol. 25 1325-1332, 1989. 

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