Liposome-encapsulated hemoglobin

Fig. 3.21. Retention rate of liposome-encapsulated hemoglobin as a function of the time elapsed after the reconstitution of the freeze dried LEH, with different trehalose concentrations as CPA. 1, no trehalose 2, 10 mM 3, 50 mM 4, 150 mM 5, 300 mM trehalose (Fig. 2 from [3.43]).

Liposome-Encapsulated Hemoglobin as an Artificial Oxygen Carrier... 

Figure 1 Schematic illustration of liposome-encapsulated hemoglobin. Abbreviations-. PEG, polyethylene gylcol HB, hemoglobin.

Figure 3 Manufacturing scheme for liposome-encapsulated hemoglobin (LEH). Lipid phase is mixed with hemoglobin and the mixture is homogenized in an extruder or a microfluidizer. Unencapsulated hemoglobin is separated by filtration, before PEGylation is performed by postinsertion. The resulting PEG-LEH is converted into oxyhemoglobin form and concentrated to obtain final product. Abbreviation IXC, interaction chamber.

Figure 4 A set of gamma camera images of a rabbit intravenously injected with Tc-99m-LEH. Twenty-five percent of blood was exchanged with liposome-encapsulated hemoglobin (LEH) (hypovolemic) and the animal was imaged using a gamma camera at various times after infusion. The images clearly show a prolonged circulation of LEH as evidenced by the continued grayscale intensity in heart.

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